用醛化红细胞分离抗禽流感病毒独特型多克隆抗体  

Isolation of polyclonal anti-idiotypic antibodies to AIV by aldehydated erythrocyte

作　　者：李宝全[1] 彭军[1] 尹逊河[1] 高强[1] 牛钟相[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医科技》2005年第7期512-514,共3页Chinese Journal of Veterinary Science and Technology

摘　　要：用纯化的鸡抗禽流感病毒(AIV)抗体免疫家兔,制备抗独特型多克隆抗体;用戊二醛醛化的红细胞固定非特异性鸡抗体,再吸附兔抗鸡抗体,获得了纯的抗独特型抗体。经鉴定,该抗体既有弱的血凝性又有弱的血凝抑制性,并能与原病毒竞争性地和鸡抗AIV抗体结合。Polyclonal anti-idiotypic antibodies to AIV were prepared from rabbits vaccinated with purified chicken anti-AIV antibodies, and isolated by aldehydated chicken red blood cells to bind unspecific chicken antibodies and to absorb rabbit-anti-chicken antibodies. The acquired antibodies were capable of agglutinating chicken red blood cells and inhibiting AIV in hemagglutinating weekly, and competed original AIV in binding chicken anti-AIV antibodies.

关 键 词：禽流感病毒 抗独特型抗体 醛化红细胞 

分 类 号：S855.3[农业科学—临床兽医学] Q813[农业科学—兽医学]