cDNA Array Analysis of the Differential Expression Change in Virulence-related Genes During the Development of Resistance in Candida albicans  

作　　者：ZhengXU Yong-BingCAO Jun-DongZHANG Ying-YingCAO Ping-HuiGAO De-JunWANG Xu-PingFU KangYING Wan-ShengCHEN Yuan-YingJIANG 

机构地区：[1]DepartmentofPharmacologySchoolofPharmacy,SecondMilitaryMedicalUniversity,Shanghai200433,China [2]StateKeyLaboratoryofGeneticEngineering,InstituteofGenetics,SchoolofLifeScience,FudanUniversity,Shanghai200433,China

出　　处：《Acta Biochimica et Biophysica Sinica》2005年第7期463-472,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China (No.30200012,No.30300442);the Key Program of Science and Technique Foundation of Shanghai (No.03JC14006)

摘　　要：Candida albicans is the most frequently isolated fungus in immunocompromised patients associated with mucosal and deep-tissue infections.To investigate the correlation between virulence and resistance on a gene expression profile in C.albicans,we examined the changes in virulence-related genes during the development of resistance in C.albicans from bone marrow transplant patients using a constructed cDNA array representing 3096 unigenes.In addition to the genes known to be associated with azole resistance, 16 virulence-related genes were identified,whose differential expressions were newly found to be associated with the resistant phenotype.Differential expressions for these genes were confirmed by RT-PCR independently.Furthermore,the up-regulation of EFG1,CPH2,TEC1,CDC24,SAP10,ALS9,SNF1,SPO72 and BDF1,and the down-regulation of RAD32,IPF3636 and UBI4 resulted in stronger virulence and invasiveness in the resistant isolates compared with susceptible ones.These findings provide a link between the expression of virulence genes and development of resistance during C.albicans infection in bone marrow transplant (BMT) patients,where C.albicans induces hyphal formation and expression change in multiple virulence factors.Candida albicans is the most frequently isolated fungus in immunocompromised patients associated with mucosal and deep-tissue infections.To investigate the correlation between virulence and resistance on a gene expression profile in C.albicans,we examined the changes in virulence-related genes during the development of resistance in C.albicans from bone marrow transplant patients using a constructed cDNA array representing 3096 unigenes.In addition to the genes known to be associated with azole resistance, 16 virulence-related genes were identified,whose differential expressions were newly found to be associated with the resistant phenotype.Differential expressions for these genes were confirmed by RT-PCR independently.Furthermore,the up-regulation of EFG1,CPH2,TEC1,CDC24,SAP10,ALS9,SNF1,SPO72 and BDF1,and the down-regulation of RAD32,IPF3636 and UBI4 resulted in stronger virulence and invasiveness in the resistant isolates compared with susceptible ones.These findings provide a link between the expression of virulence genes and development of resistance during C.albicans infection in bone marrow transplant (BMT) patients,where C.albicans induces hyphal formation and expression change in multiple virulence factors.

关 键 词：Candida albicans cDNA array RESISTANCE VIRULENCE 

分 类 号：R379.4[医药卫生—病原生物学]