圣和散对胃癌SGC-7901细胞Bcl-2表达的影响  

Effect of Shenghe Powder on expression of Bcl-2 in gastric carcinoma cells

作　　者：王建华 夏跃胜 吴永忠[2] 刘星[3] 

机构地区：[1]总后勤部第五职工医院肿瘤科,山西永济044500 [2]第四军医大学唐都医院,陕西西安710038 [3]山西中医学院,山西太原030024

出　　处：《实用医药杂志》2005年第7期621-622,共2页Practical Journal of Medicine & Pharmacy

基　　金：山西省科技发展计划项目(编号:002059)

摘　　要：目的研究中药圣和散对胃腺癌细胞SGC-7901Bcl-2表达的影响。方法圣和散作用于胃腺癌细胞SGC-7901,48h后收集细胞爬片,用抗Bcl-2mAb和免疫组织化学SABC法及图像分析技术分析Bcl-2。结果圣和散作用的胃腺癌细胞Bcl-2表达量A值120.13±0.18,较对照组细胞104.536±0.091下降显著(P<0.05)。结论圣和散对胃癌细胞的凋亡诱导作用可能与其影响Bcl-2表达有关。Objective To investigate effect of the Shenghe Powder (SHP)on expression of Bcl-2 in gastric carcinoma cell.Methods Gastric carcinoma cell line SGC-7901 were co-cultured with SHP.After 48 hours,the cells sticking to the wall of glass were collected and studied with the monoclonal antibody against Bcl-2 by using immunohistochemical SABC method and imaging analysis.Results The expression of Bcl-2 in gastric carcinoma cells co-cultured with SHP(A=120.13±0.18) decreased comparing with the controls (A=104.536±0.091) with significant difference(P<0.05). Conclusion The apoptosis induction of gastric carcinoma cells by SHP may be related with change of expression of Bcl-2.

关 键 词：圣和散 胃癌 BCL-2 

分 类 号：R285.5[医药卫生—中药学] R329.26[医药卫生—中医学]