偶发分枝杆菌MF2和MF96生物转化差异的机理研究  

Mechanism study on difference of biotransformation between Mycobacterium fortuitum MF2 and MF96

作　　者：凌良飞[1] 戈梅[2] 付磊[3] 黄为一[1] 陈代杰[2] 

机构地区：[1]南京农业大学微生物学系,南京210095 [2]上海医药工业研究院 [3]中国药科大学药学院,南京210009

出　　处：《微生物学报》2005年第4期526-529,共4页Acta Microbiologica Sinica

基　　金：国家"十五"科技攻关项目(2003AA2Z3149)~~

摘　　要：偶发分枝杆菌(Mycobacteriumfortuitum)亲株(MF2)和突变株(MF96)存在生物转化差异,通过静息细胞系统转化研究发现在反应达到平衡后,亲株中产物大部分以雄甾烯二酮(△4-androstenedione,4AD)的形式存在,而突变株中产物大部分为睾酮(testosterone,TS)。为了研究二者的转化差异,采用无细胞系统转化的手段进行了比较研究,结果表明在添加足量的NAD+和NADH后,亲株和突变株的转化产物比例基本相同。由此推测在静息细胞中的转化产物比例不同可能是由于辅酶NAD+与NADH的比例不同引起的。最后通过测定亲株和突变株中辅酶NAD+和NADH的比例证实该推测是正确的。Biotransformation difference between parent strain (MF2) and mutant strain (MF96) of Mycobacterium fortuitum was observed. Biotransformation with resting cells showed that the major products of biotransformation by both parent and mutant strains are △ 4 -androstenedione(4AD) and testosterone(TS). Experiments with cell-free extract system showed that the proportion of 4AD/TS obtained from parent and mutant strains was almost same when enough NAD + and NADH were supplied in this system. It was suggested that the difference of the ratio of products transformed by both strains in resting cell system may result from their different ratio of NAD + /NADH. This speculation was verified to be true by determination of the amount of NAD + and NADH presented in both strains.

关 键 词：17β-甾体羟基脱氢酶 辅酶 生物转化 

分 类 号：Q935[生物学—微生物学]