恶臭假单胞菌NA-1菌株烟酸羟基化酶活性的诱导和转化条件的研究  

Induction of nicotinic acid hydroxylase activity of Pseudomonas putida NA-1 and optimization of transformation conditions

作　　者：陆伟宏[1] 王鑫[1] 徐莉[1] 戴亦军[1] 袁生[1] 

机构地区：[1]南京师范大学生命科学学院江苏省资源生物技术重点实验室南京师范大学微生物工程重点实验室,南京210097

出　　处：《微生物学报》2005年第4期551-555,共5页Acta Microbiologica Sinica

基　　金：国家"十五"科技攻关项目(2001BA308A05-02)~~

摘　　要：恶臭假单胞菌NA-1菌株的培养和产酶特性与已报道的产酶菌株粘质沙雷氏菌(Serratiamarcescens)IFO12648和荧光假单胞菌(Psudomonasfluorescens)TN5有所不同,主要反映在最适碳源及浓度、最适诱导剂浓度和最适培养温度等方面。最适的转化条件是温度为30℃,pH为7.0,烟酸的浓度为3%。采用初步优化后的条件和流加底物的方式进行4L上罐生产,恶臭假单胞菌NA-1菌株的6-羟基烟酸产率可达到108.39gL。Some features of Pseudomonas putida NA-1 strain in cultivation and enzyme production were different from S. marcescens IFO 12648 and P. fluorescens TN5 stains which could transform nicotinic acid to 6-hydroxynicotinic acid reported by other scientists, such as optimal carbon and its optimal concentration, optimal inducer concentration, and optimal cultivation temperature. The ideal transformation condition was nicotinic acid 3%, temperature 35℃ and pH 7.0.Under an appropriate condition, in a 4 liter fermenter, production yield of 6-hydroxynicotinic acid by Pseudomonas putida NA-1 was 108.39g/L.

关 键 词：烟酸 6-羟基烟酸 生物转化 恶臭假单孢菌 

分 类 号：Q819[生物学—生物工程]