阳虚模型小鼠腹腔巨噬细胞Ia抗原表达及乌头碱的作用  

Ia Antigen Expression of Peritoneal Macrophage in Mice Model of Yang Deficiency and the Effect of Aconitine

作　　者：马健[1] 樊巧玲[1] 

机构地区：[1]南京中医药大学

出　　处：《中国中西医结合杂志》1995年第9期544-546,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：巨噬细胞Ｉａ抗原表达是衡量机体特异性免疫功能强弱的重要指标之一。本研究观察了皮质酮后腿肌肉注射复制的小鼠下丘脑─垂体─肾上腺轴抑制的“阳虚”动物模型腹腔巨噬细胞表面Ｉａ抗原表达的改变，及温阳代表药物附子的主要成分乌头碱对其的作用。结果表明：阳虚动物腹腔巨噬细胞对γ－干扰素（ＩＦＮ－γ）诱导的Ｉａ表达活性较正常对照组降低（Ｐ＜０．０１）；乌头碱则能明显提高阳虚模型小鼠巨噬细胞Ｉａ抗原表达的水平（Ｐ＜０．０１）。提示阳虚证免疫功能低下的机理，可能与阳虚状态时巨噬细胞提交抗原能力降低有关；乌头碱增强巨噬细胞表面Ｉａ抗原表达，提高其提交抗原能力，从而促进免疫应答反应，可能是附子提高免疫功能、治疗阳虚证的作用机理之一。The immune associated(Ia)antigen expression(IAAE) of macrophage is one of the important indicators of the specific immunity in the body.In this paper,mice of Yang Deficiency model was made by injecting corticosterone in the hind legs of mice to inhibit the function of hypothalamus -pituitary-adrenal axis.Authors observed the changes of IAAE in model mice and the effect of Aconitine on it.The results indicated that:(1)In model mice,the IFN-γ induced IAAE of peritoneal macrophages was inhibited.The difference of IAAE between the model and the normal mice was significant(P<0.01).(2)Aconitine could significantly increase the IAAE of model mice(P<0.01).These data implied that the immuno-suppression in the model mice of Yang Deficiency might be due to the lowered IAAE of macrophages under this condition.The promoting effects of Aconitine on IAAE enhanced the antigen presenting ability of macrophages and the immune response of the model mice.It might be related to the therapeutic mechanism of Aconitine on Yang Deficiency Syndrome.

关 键 词：阳虚 皮质酮 动物模型 巨噬细胞 IA抗原表达 

分 类 号：R255.5[医药卫生—中医内科学]