甲状腺素对胃腺癌细胞VLDL-R选择性剪接的影响  

Effect of Thyroxine on the Alternative Splicing of Very Low Density Lipoprotein Receptor in Gastric Adenocarcinoma Cells

作　　者：杨璞[1] 李映红[1] 田俊[1] 陈涛[1] 宗义强[1] 屈伸[1] 

机构地区：[1]华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市430030

出　　处：《医学分子生物学杂志》2005年第4期259-261,共3页Journal of Medical Molecular Biology

基　　金：国家自然科学基金(No.30470872)~~

摘　　要：目的探讨甲状腺素对低分化胃腺癌细胞MKN45细胞中极低密度脂蛋白受体(VLDL-R)选择性剪接的影响。方法在体外,用一定浓度的甲状腺素与MKN45细胞温育不同时间后,采用RT-PCR和Western印迹技术检测两型VLDL-R mRNA和蛋白质的表达水平,以两型受体的比值间接反映甲状腺素对VLDL-R选择性剪接的影响。结果甲状腺素可明显上调两型VLDL-R的表达,Ⅱ型受体与Ⅰ型受体mRNA的比值随时间延长逐渐增大。结论甲状腺素可显著增强VLDL-R的选择性剪接,表现为Ⅱ型受体表达的增高幅度明显大于Ⅰ型受体。Objective To study the effect of thyroxine on the alternative splicing of very low density lipoprotein receptor in gastric adenocarcinoma cells (MKN45 cells).Methods MKN45 cells were incubated with 10nmol/L of thyroxine for 4, 8, 12, 24 h respectively.The expression of mRNA of the two variants of VLDL receptor was examined by RT-PCR and its expression of protein was examined by Western blotting.Results Thyroxine up-regulated the mRNA and protein expression of two isoforms of VLDL-R in a certain time range, and increased the ratio of type Ⅱ VLDLR mRNA to type Ⅰ VLDLR mRNA over time.Conclusion Thyroxine can obviously increase VLDLR alternative splicing.

关 键 词：VLDL-R 选择性剪接 甲状腺素 胃腺癌细胞 MKN45细胞 极低密度脂蛋白受体 Western印迹 RT-PCR 受体mRNA 不同时间 技术检测 时间延长 Ⅱ型受体 Ⅰ型受体 受体表达 低分化 蛋白质 比值 

分 类 号：R587.102[医药卫生—内分泌] R384.2[医药卫生—内科学]