补肾密骨液作用于卵巢切除大鼠的骨组织计量学研究  

Histomorphometric Study of Bu Shen Mi Gu Ye(BSMGY)on Ovariectomized Rats

作　　者：谢晶[1] 杜靖远[1] 沈霖[1] 

机构地区：[1]同济医科大学附属协和医院骨科研究室

出　　处：《中国中医骨伤科》1995年第6期1-3,8,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：为探讨补肾密骨液预防绝经后骨质疏松症的作用及机理，选用４０只８月龄雌性Ｗｉｓｔａｒ大鼠随机分为：假手术组、卵巢切除组、卵巢切除后用雌二醇组、卵巢切除后用补肾中药组和卵巢切除后用盐水组。用药均在卵巢切除后第二天进行。１０周后，取大鼠胫骨近端制成不脱钙骨切片行骨组织形态计量学测定。结果显示，卵巢切除后大鼠小梁骨体积、矿化小梁骨体积降低，表明绝经后骨质疏松症动物模型成立。补肾密骨液组其小梁骨体积与矿化小梁骨体积明显高于盐水组，而其反映骨形成，矿化和骨吸收的指标均下降，表明补肾密骨液可抑制升高的骨转换，且抑制吸收强于抑制形成，进而使骨转换降低而防止骨量丢失。In order to study the effect of BSMGY in prevention of osteoporosis,we selected 40 female Wistar rats at 8 months old subjected to following groups; sham surgery,Bilateral Ovariectomized(OVX),OVX treated with estradiol,OVX treated with BSMGY and OVX treated with saline.All drugs were used one day after OVX.Ten weeks later,proximal tibae were proceeded undecalcified for quantitative bone histoafter morphometry.The results show the cancellous bone volume(CBV),mineral cancellous bone volume(mCBV)after OVX were markedly low which proves the model of postmenopausal osteoporosis.Group BSMGY characterized with higher CBV and mCBV,but lower indices of boneturn-over,It proves BSMGY can prevent bone loss in ovariectomized rats by reducing higher bone turn-over especially bone resorption.

关 键 词：中药 补肾密骨液 卵巢切除 骨质疏松 骨组织计量 

分 类 号：R285.5[医药卫生—中药学] R274.91[医药卫生—中医学]