家蚕抗核型多角体病毒病分子标记辅助育种  

Molecular Markers-assisted Breeding for Silkworm Resistant Variety to Bm NPV

作　　者：姚勤[1] 刘晓勇[1] 唐旭东[1] 陈克平[1] 

机构地区：[1]江苏大学生命科学研究院,镇江212018

出　　处：《分子植物育种》2005年第4期537-542,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(30370773);江苏高校高新技术产业发展面上项目(JH0—034)资助。

摘　　要：利用我国家蚕种质资源中发现的高抗NPV的材料NB和敏感材料306,组配近等基因系。采用RAPD技术获得分子标记,将标记转换成SCAR(sequencecharacterizedamplifiedregion)标记,利用SCAR标记开展了家蚕抗NPV新品种辅助育种选择,获得了家蚕抗NPV新品种。The isogenic line was constructed between the highly resistant strain and the susceptive strain of germplasm stock of silkworm in China. After molecular marker was obtained by RAPD technology, it was converted to SCAR (sequence characterized amplified region) marker. New silkworm strain which is resistant to NPV was obtained in the process of molecular marker assisted breeding based on the SCAR marker.

关 键 词：分子标记辅助育种 核型多角体病毒病 家蚕 抗NPV RAPD技术 SCAR标记 近等基因系 敏感材料 种质资源 育种选择 新品种 组配 

分 类 号：S882.3[农业科学—特种经济动物饲养]