壬基酚对大鼠F1代脑物质能量代谢调控相关基因表达的影响  

EFFECTS OF NONYLPHENOL ON BRAIN SUBSTANCE AND ENERGY METABOLISM ASSOCIATED GENE EXPRESSION PROFILES OF F1 GENERATION RATS

作　　者：姚永革[1] 詹平[1] 李元锋[1] 

机构地区：[1]重庆医科大学预防医学系环境卫生学教研室,重庆400016

出　　处：《现代预防医学》2005年第8期874-876,共3页Modern Preventive Medicine

基　　金：国家教育部西部高校高级访问学者基金(20023)

摘　　要：目的:利用芯片技术探讨壬基酚(nonylphenol,NP)对F1代脑物质能量代谢调控相关基因表达影响。方法:NP染毒F0亲代母鼠,分别提取NP组和对照组F1代雄性2日龄大鼠的脑组织mRNA,分别用cy5和cy3逆转录标记,与BiostarR-40S基因表达谱芯片杂交,并对cy3、cy5荧光信号做扫描分析。结果:芯片结果显示实验组已知功能差异表达基因有16条,其中下调基因有13条,5条与物质能量代谢调控密切相关。结论:NP可通过多种途径影响机体物质能量代谢功能。Objective:To explore the effects of nonylphenol on brain substance and energy metabolism associated gene expression profiles of F1 generation rats by means of microarray technique.Methods:The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol,then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence.Subsequently,the cDNA probes were hybridized to the BiostarR-40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scaned and analyzed.Results:16 identified genes expressed differently,including 13 down-regulated in which 5 were related to substance and energy metabolism.Conclusion:Nonylphenol can effect the function of substance and energy metabolism of the male rats.

关 键 词：壬基酚 基因表达谱芯片 物质能量代谢调控相关基因 

分 类 号：R114[医药卫生—卫生毒理学]