高效液相色谱法测定红毛五加中绿原酸的含量(英文)  

HPLC Determination of Chlorogenic Acid in Acanthopanax giraldii Harms

作　　者：王祝伟[1] 张庆海[1] 张莅峡[2] 孙毓庆[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]中国中医研究院,北京100700

出　　处：《药物分析杂志》2005年第7期765-768,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用LC/MS2方法鉴定出中药红毛五加中绿原酸成分,并用高效液相色谱法测定其含量。方法:采用Zorbax SB-C18色谱柱(150 mm×4.6mm,5μm);流动相为乙腈和水(含0.16%甲酸)二元梯度洗脱系统;流速为1.0mL·min-1,检测波长327 nm。质谱条件:Agilent多级离子阱质谱仪:电喷雾化学电离源(ESI);负离子检出模式。结果:绿原酸的线性范围为 10-100 mg·L-1(r=0.9997),加样回收率为99.2%-101.8%;红毛五加中绿原酸的含量为0.58-5.45mg.g-1。结论:本方法灵敏、快速、重现性好,有助于红毛五加质量控制方法的研究。Objective:Chlorogenic acid in Acanthopanax giraldii Harms was identified by LC/MS . Method:RP -LC method was applied for the determination of chlorogenic acid in Acanthopanax giraldii Harms collected in Sichuan province,China. The separation was performed on a Zorbax SB C18( 150 mm×4.6 mm,5μm) column,and a mobile phase program of gradient elution with acetonitrile and 0.16% aqueous formic acid at a flow rate of 1.0mL·min-1 was employed. The column effluent was monitored at UV 327 nm with diode array detector (DAD). The mass spectrometry analysis was performed on the Agilent 1100 series ion trap mass spectrometer with an electrospray ionization source in the negative ion detection mode. Qualitative identification was carried out by the comparison of the HPLC retention time and the LC/MS2 spectra of chlorogenic acid with corresponding authentic sample. Results:The linear range of chlorogenic acid was 10-100 mg·L-1 (r =0.9997) and the average recovery was 99.2%-101.8% . The content of chlorogenic acid in A. giraldii Harms was 0. 58 -5. 45 mg·g-1. Conclusion:The method is sensitive and rapid, with a good reproducibility. It is useful for the quality control of A. giraldii Harms.

关 键 词：高效液相色谱法 红毛五加 中绿原酸 含量 测定 AGILENT C18色谱柱 质量控制方法 加样回收率 梯度洗脱 检测波长 化学电离 质谱条件 检出模式 线性范围 流动相 电喷雾 质谱仪 负离子 重现性 

分 类 号：R286.0[医药卫生—中药学] R285.5[医药卫生—中医学]