反相高效液相色谱法测定蛇床子中蛇床子素的含量  

RP-HPLC Determination of Osthole in Fructus Cnidii

作　　者：吴波[1] 马长清[2] 张寒俊[1] 

机构地区：[1]武汉工业学院湖北省粮食加工与转化重点实验室,武汉430023 [2]华中科技大学同济医学院药学院药物分析系,武汉430030

出　　处：《药物分析杂志》2005年第7期843-845,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立反相高效液相色谱法对蛇床子中蛇床子素的分析方法。方法:色谱柱为BDSHYPERSIL C18柱(250 mm× 4.6 mm,5μm);流动相:乙醇-水(60:40);流速1 mL·min-1,荧光检测器:激发波长:357 nm发射波长:395 nm。结果:在选定色谱条件下其蛇床子素的线性范围良好,样品加样回收率为100.6%,RSD为1.8%。结论:该方法测定快速,结果准确、可靠。Objective:To develop a quantitative method for determination of osthole in Fructus Cnidii by RP-HPLC. Method:BDSHYPERSIL C18 column(250 mm×4.6 mm,5μm) was used.ethanol-water(60:40) as the mobile phase,flow rate at 1 mL·min-1, fluorescence detector:λex357 nm,λex395 nm. Results:The good linear relationship was obtained under the optimum conditions. The recoveries of the standards added was 100.6% while the relative standard deviations was 1.8%.Conclusions:The method is rapid and precise.

关 键 词：反相高效液相色谱法 蛇床子素 测定 含量 荧光检测器 加样回收率 线性范围 色谱条件 色谱柱 流动相 RSD 波长 

分 类 号：R927.2[医药卫生—药学] R286.0