胶束电动毛细管色谱法手性拆分那格列奈  

Enantioseparation of Nateglinide by Micellar Electrokinetic Chromatography

作　　者：黄月君[1] 刘力宏[2] 杜国华[3] 谢剑炜[3] 

机构地区：[1]山西生物应用职业技术学院,太原030031 [2]厦门大学化学化工学院化学系 [3]军事医学科学院毒物药物研究所,北京100850

出　　处：《药物分析杂志》2005年第7期852-855,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:选择合适的环糊精作手性选择剂,建立那格列奈对映体的胶束电动毛细管色谱拆分方法。方法:使用Tris- H3PO4缓冲液研究了运行液pH、背景电解质浓度、表面活性剂浓度、环糊精种类和浓度及有机添加剂对分离的影响,确定了拆分那格列奈的最佳实验条件:200 mmol·L-1Tris-H3PO4缓冲液,pH=7.8,含40 mmol·L-1 SDS,5 mmol·L-1 HP-β-CD, 10%(v/v)正丙醇,检测波长215 nm。结果:在所建立的最佳条件下,那格列奈对映体达到基线分离,分离度为1.68。结论:方法简单、快速,可作为那格列奈的手性分离方法。Objective:To develop a simple, rapid method for enantioseparation of nateglinide by cyclodextrin (CD)-modified micellar electrokinetic chromatography (MEKC), Method:Using Tris-H3PO4 buffer solution, 215 nm as the detective wavelength, the influence of several factors, such as Tris concentration, pH, anionic surfactant sodium dedecyl sulfate (SDS) concentration, CDs types and their concentration, and organic additive, on the enantimeric separation of nateglinide was investigated and the optimal condition for the separation was established. The buffer solution was 200 mmol·L-1 Tris - H3PO4 at pH 7. 8 containing40mmol·L-1 SDS,5 mmol·L-1 HP-P-CD and 10% (v/v)n-propanol. Result:Under the experiment conditions adopted, the baseline separation of nateglinide enantiomers was obtained, with the resolution 1.68. Conclusion:The proposed method is simple and can be used for the chiral separation of nateglinide.

关 键 词：胶束电动毛细管色谱法 那格列奈 手性拆分 HP-β-CD 最佳实验条件 手性选择剂 电解质浓度 表面活性剂 拆分方法 检测波长 最佳条件 基线分离 分离方法 环糊精 对映体 缓冲液 添加剂 正丙醇 分离度 

分 类 号：R978.5[医药卫生—药品] R977.15[医药卫生—药学]