基因枪转化小麦愈伤组织的影响因素研究  

Factors of gene transformation affecting wheat callus

作　　者：曹团武[1] 朱建楚[2] 亢福仁[1] 陈耀锋[1] 曹鑫[1] 赵云祥[1] 任慧莉[1] 李春莲[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]榆林学院,陕西榆林719000

出　　处：《西北农林科技大学学报（自然科学版）》2005年第7期65-67,72,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家转基因专项(JY03-B-23-02);陕西省科技攻关项目(2003K03-G1-04)

摘　　要：以小麦愈伤组织为受体材料,用基因枪法将报告基因GUS和目的基因Psag12-IPT导入小麦中。GUS基因表达检测表明,金粉用量越高,转化频率越高;质粒DNA用量以2.0μg/枪最佳;随着愈伤组织培养时间的延长,GUS基因瞬时表达呈明显下降趋势,至第28天以后趋于稳定。另外,GUS基因瞬时表达检测应在24h内进行。The plamid pcMA35-1 containing both GUS gene and Psag12-IPT gene was driven by CAMV35S promoter into wheat callus using ballistic method.Parameters affecting transformation efficiency were studied by GUS transient expression.The result showed that it had a remarkable role in the transformation efficiency at a relatively high gold particle amount per bombardment and it was best when coated by 2.0 μg DNA.The expression efficiency of GUS gene reduced with the increase of callus culture time and remained stable after 28 d.The examination of GUS gene transient expression should be at 1 d and the examination of GUS gene stable expression should be after 28 d.

关 键 词：愈伤组织 基因枪转化 小麦 Psag12-IPT 报告基因GUS GUS基因 瞬时表达 质粒DNA 受体材料 目的基因 基因枪法 基因表达 转化频率 培养时间 检测 用量 

分 类 号：S511.210.3[农业科学—作物学] Q943.1[生物学—植物学]