冠心病体质表型和低密度脂蛋白受体基因Avall位点多态性关系的研究  

Study on Relationship Between the Polymorphisms of Low Density Lipoprotein Receptor gene and the Constitutions in Patients with Coronary Heart Disease

作　　者：欧阳涛[1] 宋剑南[1] 林谦[2] 李林[3] 苗阳[4] 牛晓红[1] 金红[1] 陈冰[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700 [2]北京中医药大学东方医院,北京100078 [3]北京大学数学科学学院,北京100871 [4]中国中医研究院西苑医院,北京100091

出　　处：《中国中医基础医学杂志》2005年第7期521-523,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(30171128);国家重点基础研究发展计划(2003CB517105)

摘　　要：目的:探讨CHD(冠心病)体质类型和LDLR(低密度脂蛋白受体)第13外显子AvaⅡ位点基因多态性的关系。方法:选择符合条件的冠心病中医痰证、瘀证、痰瘀互阻证和非痰非瘀证(其他证型)患者200例,判别患者的体质类型。另选100例健康对照,常规检测所有样品的血脂水平。提取全血DNA,经PCR扩增获取目的片段,用AvaⅡ酶切检测LDLR基因型,采用SAS软件进行相关性分析。结果:患者的-+基因型显著多于对照。TC(总胆固醇)、TG(总甘油三酯)和LDLC(低密度脂蛋白胆固醇)水平在--、-+、++基因型患者中存在渐升的趋势,++基因型的TC水平和动脉硬化指数AI显著高于--基因型。患者的体质类型以瘀血质、痰湿质和气虚质多见,其中痰湿质和+等位基因,瘀血质与++基因型密切相关。结论:瘀血质、痰湿质、气虚质是冠心病的主要体质表型。LDLR第13外显子AvaⅡ位点+等位基因的携带患者多出现在痰湿质和瘀血质中,纯合子患者的TC和AI升高。这为痰瘀互结的中医病机研究提供了初步证据。Objective:Aim is to explore the relationship between the polymorphism of LDL-R gene exon 13 AvaⅡ site and the constitutions of CHD patients.Methods:Differentiated by Syndrome Differentiation-type standards(TCM-SDT),200 CHD patients involved Phlegm syndrome (PS),blood stasis syndrome (BSS),Phlegm-Blood Turbid syndrome (PBTS),Non-Phlegm-Non-Blood stasis syndrome (NPNBS) were adopted.The constitutions of patients were judged according to “The theory of TCM constitutions”^([1]).The controls are 100 healthy cases.Blood lipids were measured by routine examine.The peripheral blood total DNA were extracted.The LDL-R polymorphisms were determined firstly by using PCR to amplify the DNA sequence containing the polymorphisms site,followed by digestion with the restriction enzyme AvaⅡ.The relativity of data was analyzed by SAS software.

关 键 词：冠心病 中医体质 低密度脂蛋白受体 基因多态性 证候 

分 类 号：R259.414[医药卫生—中西医结合]