nm23基因产物、细胞外基质抗原表达与喉癌颈淋巴结转移关系的研究  

Relationships between the expression of nm23/NDPK, extracellular matrix antigen and the metastasis of cervical lymph node in laryngeal squamous cell carcinomas

作　　者：曹志伟[1] 施磊[1] 李笑天[1] 任重[1] 

机构地区：[1]中国医科大学第一附属医院耳鼻喉科,辽宁沈阳110001

出　　处：《山东大学基础医学院学报》2005年第4期205-208,F003,共5页Journal of Preclinical Medicine College of Shandong Medical University

基　　金：辽宁省自然科学基金资助课题(9522019)

摘　　要：目的:探讨nm23基因产物和细胞外基质抗原的表达与声门上型喉鳞癌颈淋巴结转移的关系。方法:分别应用nm23/NDPK多克隆抗体、抗Ⅳ型胶原纤维(typeIVcollagen,CTⅣ)单克隆抗体、抗层黏连蛋白(laminin,LN)单克隆抗体、抗纤维连接蛋白(fibronectin,FN)单克隆抗体以链霉素亲生物素—过氧化酶免疫组织化学法观察nm23/NDPK和这3种抗原在72例声门上型喉鳞癌中的表达与分布及其与颈淋巴结转移的关系。统计学处理采用χ2检验。结果:nm23/NDPK阴性者颈淋巴结转移率高于表达阳性者,二者之间有较强的相关性(P<0.01)。CTⅣ、LN、FN表达缺失者发生颈淋巴结转移率高于表达完整者,也表明其间有较强的相关性(P<0.01)。结论:nm23/NDPK、细胞外基质3种抗原的表达可作为预测声门上型喉鳞癌发生颈淋巴结转移的重要参数之一。Objective: To investigate the relationships between the expression of nm23/NDPK, extracellular matrix (ECM) and the metastasis of cervical lymph node in laryngeal squamous cell carcinomas. Methods: An immunohistochemical study was performed using the polyclonal antibody against nm23/NDPK and three monoclonal antibodies against human typeⅣcollagen (CTⅣ),laminin (LN) and fibronectin (FN) to observe the expression and the distribution of nm23/NDPK and extracellular matrix (ECM) antigens in 72 cases of supraglottic laryngeal squamous cell carcinomas,and to assess the relationship with the metastasis of cervical lymph node. χ 2 test was applied for statistic analysis. Results: There was a higher risk of cervical lymph node metastasis among cases with negative expression of nm23/NDPK and defect ECM expression than those with positive nm23/NDPK expression and continual ECM expression. There was a significant association between them (P<0.01). Conclusion: These observations indicate that testing the distribution and the expression of nm23/NDPK and ECM seems to be helpful to prognosticate the frequency of cervical lymph node metastasis in laryngeal squamous cell carcinomas.

关 键 词：喉肿瘤 肿瘤 鳞状细胞 基因 nm23 细胞外基质蛋白质类 肿瘤转移 

分 类 号：R739.65[医药卫生—肿瘤]