组织蛋白酶L在扩张型心肌病心肌中的表达及其意义  

The expression and significance of myocardial cathepsin L in dilated cardiomyopathy

作　　者：于小华[1] 张新刚[2] 李双杰[1] 王时俊[2] 赵刚[2] 陈瑞珍[2] 杨英珍[2] 

机构地区：[1]湖南衡阳市南华大学附属第一医院儿科 [2]复旦大学附属中山医院上海市心血管病研究所卫生部病毒性心脏病重点实验室

出　　处：《中华内科杂志》2005年第7期495-498,共4页Chinese Journal of Internal Medicine

基　　金：国家自然科学基金资助项目(30271665)

摘　　要：目的探讨溶酶体组织蛋白酶L在扩张型心肌病(DCM)心肌组织中的表达及其在DCM发病中的意义。方法终末期扩张型心肌病行心脏移植术的受体标本20例(心肌病组)及脑死亡3h内排除心脏疾患的心脏标本5例(对照组),通过免疫组化、Westernblotting和实时定量RTPCR等方法检测心肌组织组织蛋白酶L基因转录及蛋白表达水平,并对组织蛋白酶LmRNA表达水平的动态变化与射血分数进行相关性分析。结果心肌病组心肌中组织蛋白酶LmRNA及蛋白表达水平均明显高于对照组,两组比较,差异有高度统计学意义(P值均<0.01);DCM患者心肌组织蛋白酶LmRNA表达水平与射血分数呈显著负相关(r=-0.884,P<0.01)。结论组织蛋白酶L过度激活可能在扩张型心肌病的发病机制中起重要作用。Objective To clarify the expression of myocardial cathepsin L and its significance in the pathogenesis of dilated cardiomyopathy (DCM).Methods Myocardial tissue specimens derived from 20 patients undergoing orthotopic cardiac transplantation and 5 normal myocardium samples collected at autopsy from sudden death as controls were studied. The expression of cathepsin L was detected with immunohistochemistry,real time PCR and Western Blotting, respectively. At the same time,the relationship between the Cathepsin L mRNA expressional levels and the myocardial function(ejection fraction,EF) was investigated.Results The expression levels of cathepsin L mRNA and protein in DCM group were markedly elevated compared with those in control group and the expression levels of cathepsin L mRNA had significantly negative correlation with the myocardial function(ejection fraction,EF).Conclusion (Cathepsin) L is markedly elevated in DCM myocardial tissue and it may participate in the pathogenesis of DCM.

关 键 词：组织蛋白酶L 扩张型心肌病 基因表达 DCM 心肌组织 

分 类 号：R542.2[医药卫生—心血管疾病]