肠毒素大肠埃希菌载体活菌疫苗的安全性和免疫性研究  

Study on the safety and immunogenicity of enterotoxigenic Escherichia coli recombinant active vaccine

作　　者：王志斌[1] 曾年华[1] 姜普林[1] 李淑琴[2] 张兆山[2] 

机构地区：[1]广州军区军事医学研究所,510507 [2]北京生物工程研究所

出　　处：《中华流行病学杂志》2005年第7期515-519,共5页Chinese Journal of Epidemiology

基　　金：国家高技术研究发展计划"863计划"资助项目(2004AA215212)

摘　　要：目的研究肠毒素大肠埃希菌活菌载体疫苗FE3、FE16的安全性和免疫原性。方法通过肠毒素大肠埃希菌肠毒素毒力试验、新西兰大白兔免疫试验、小鼠口服和鼻饲途径免疫试验,检测载体疫苗的毒性、免疫原性和免疫效果。结果毒力试验中所有检测均为阴性;免疫4次后大白兔血清对福氏2a型菌的凝集效价均不低于1∶640,对肠毒素大肠埃希菌菌毛抗原的凝集效价均不低于1∶1280;通过口服和鼻饲方式免疫小鼠后,血清中IgG显著升高,同时能够在粪便中检测到分泌型IgA,而灭活苗免疫未能检测到分泌型IgA。结论活菌载体疫苗株FE3和FE16具有良好的安全性和免疫原性,同时能够刺激机体产生体液免疫和黏膜免疫反应。Objective To study the safety, immunogenicity on the enterotoxigenic Escherichia coli(E.coli ) recombinant active vaccine FE3 and FE16. Methods Toxicity and immunogenicity of the vaccine were determined by experiments on enterotoxigenic E.coli toxicity and immunological experiments on rabbits and mice. Results The results of an toxicological experiments were negative. The agglutination titer of antibodies against the S.flexneri 2a and enterotoxigenic E.coli plamid antigen were all higher than 1∶640 and 1∶1280 in the sera of rabbits. IgG in the serum went up remarkably, while sIgA against CFA/I was also decteted in the dejecta of mice immunized with active bacteria either orogastrically or intranasally. Simultaneously, sIgA was not detected in the dejecta of mice immunized with inactive bacteria either orogastrically or intranasally. Conclusion The enterotoxigenic E.coli recombinant active vaccine showed good safety and immunogenicity, inducing both humoral and mucosal immunity in mice.

关 键 词：安全性 活菌疫苗 免疫性 大肠埃希菌肠毒素 分泌型IGA 免疫原性 新西兰大白兔 黏膜免疫反应 载体疫苗 毒力试验 免疫试验 凝集效价 免疫效果 菌毛抗原 免疫小鼠 鼻饲方式 体液免疫 FE3 检测 兔血清 IgG 疫苗株 口服 

分 类 号：R392[医药卫生—免疫学]