单个红细胞的拉曼光谱研究  

Raman Spectra of Single Blood Cells

作　　者：姚辉璐 王桂文[1] 何碧娟[2] 黎永青[3] 何敏[4] 

机构地区：[1]广西科学院生物物理实验室,广西南宁530003 [2]广西科学院科研处,广西南宁530003 [3]东卡罗莱纳大学物理系,美国北卡莱罗纳278584353 [4]广西医科大学实验中心,广西南宁530021

出　　处：《济南大学学报（自然科学版）》2005年第4期328-330,共3页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金资助项目(30460121)

摘　　要：研究了不同物种的血红细胞的拉曼光谱,采用一个波长为785nm的半导体激光束来囚禁血红细胞和激发血红细胞的拉曼光谱。结果显示不同物种血红细胞的拉曼光谱在1603cm-1苯丙氨酸和色氨酸的C=C平行振动模及1616cm-1酪氨酸和色氨酸的C=C拉伸模有明显的差异,这可以用来作为不同种血红细胞鉴定的特征标记,表明激光镊子拉曼光谱技术可以成为血红细胞分析的有效手段。The Raman spectra of blood cells of different species are investigated using a laser tweezers Raman Spectroscopy (LTRS).In our system,a low-power diode laser at 785?nm was applied for both the trapping of a blood cell and the excitation of its Raman spectrum.Visual inspection of spectra shows that the differences observed in spectra of different cells in band intensities at 1?603?cm^(-1)(C=C in-plane bending mode of phenylalanine and tyrosine) and 1?616?cm^(-1)(C=C stretching mode of tyrosine and tryptophan) are obvious.This can be used as the marks for blood cell of different species.And LTRS is a useful tool for the blood cell analysis.

关 键 词：光学 显微拉曼光谱 血红细胞 激光镊子 

分 类 号：O657.37[理学—分析化学]