荧光猝灭法测定痕量亚硝酸根  

A fluorescence quenching method for the determination of nitrite

作　　者：孔继川[1] 牛建平[1] 张娜[1] 钱力[1] 樊静[1] 

机构地区：[1]河南师范大学化学与环境科学学院河南省环境污染控制重点实验室,新乡453007

出　　处：《分析试验室》2005年第7期7-9,共3页Chinese Journal of Analysis Laboratory

基　　金：河南省自然科学基金(0211020200);河南省教育厅自然科学基金(20016100005)资助

摘　　要：基于在酸性介质中,NO-2与藏红T直接反应,使藏红T的荧光猝灭,加入强碱弱酸盐对体系有增敏作用,据此提出了荧光法测定痕量NO-2的新方法。方法的线性范围为2.8～294ng/mL,方法的检出限为2.8ng/mL。可直接用于水样及蔬菜中痕量NO-2的测定。<Abstrcat>A simple and sensitive fluorescence quenching method is described for the determination of nitrite, based on the reaction of Safranine T with nitrite in acidic medium to form a new compound with lower fluorescence intensity. The addition of acetate sodium enhances the sensitivity. The detection limit is found to be 2.8 (ng/mL). The linear range of the determination is 2.8～294 ng/mL. This method was used to determine nitrite in water samples and vegetables.

关 键 词：荧光猝灭 NO^-2 藏红T 

分 类 号：O657.32[理学—分析化学]