固相萃取高效液相色谱法快速测定红豆杉枝叶中的3种紫杉烷类化合物  

Rapid determination of taxol and its analogues in taxus by solid phase extraction and high performance liquid chromatography

作　　者：雒丽娜[1] 董慧茹[1] 郑云 赵京城 

机构地区：[1]北京化工大学理学院,北京100029 [2]北京普瑞孚天然药物现代纯化和分离研究所,北京100088

出　　处：《分析试验室》2005年第7期80-84,共5页Chinese Journal of Analysis Laboratory

摘　　要：研究了用固相萃取预分离高效液相色谱法测定红豆杉枝叶中紫杉醇、三尖杉宁碱和7 表紫杉醇的方法。其中,固相萃取使用了一种新型的PRP 6高分子树脂。红豆杉枝叶经干燥粉碎后用甲醇提取,提取液用PRP 6固相萃取小柱预分离,以V(乙腈)∶V(水)=45∶55为流动相,用C18色谱柱进行分离,紫外检测器227nm测定上述3种紫杉烷类化合物,该方法加标回收率为91%～98%。此法可以快速准确的测定不同红豆杉枝叶中上述3种紫杉烷类化合物。<Abstrcat>A simple, rapid and sensitive method for the determination of Taxol and its analogues in Taxus using solid phase extraction and high performance liquid chromatography was developed in which a novel high molecular resin was applied. Powdered plant material was extracted with methanol. After being purified by PRP-6 solid phase extraction, the purified extract was analyzed directly by high performance liquid chromatography with a C_(18) column and UV detector at 227 nm. Mixtures of acetonitrile and water (45∶55 in volume ration) were used as mobile phase for elution. The recoveries were between 91% and 98%. Taxol and its analogues in plant extract were separated satisfactorily.

关 键 词：固相萃取 高效液相色谱法 紫杉醇 三尖杉宁碱 7-表-紫杉醇 

分 类 号：O658.1[理学—分析化学]