超抗原SPE对SLE患者外周血T淋巴细胞增殖和凋亡作用的研究  

Study on Proliferation and Apoptosis of Superantigen SPE on T Lymphocyte in SLE Peripheral Blood

作　　者：王红兵 [1] 杨策尧 [2] 曾卫玲 [1] 

机构地区：[1]昆明医学院第一附属医院皮肤科,云南,昆明,650031 [2]昆明医学院第一附属医院中心实验室,云南,昆明,650031

出　　处：《中国皮肤性病学杂志》2005年第7期395-397,共3页The Chinese Journal of Dermatovenereology

基　　金：云南省卫生厅科学研究基金(99Q028)

摘　　要：目的研究超抗原SPE对SLE患者外周血T淋巴细胞增殖及凋亡的作用。方法用SPE刺激SLE患者外周血T淋巴细胞,用流式细胞仪观察T淋巴细胞增殖、细胞表型以及凋亡情况,ELISA法检测IL2产生。结果SPE有促进SLE患者外周血T淋巴细胞增殖的作用,增殖细胞主要为CD4+T淋巴细胞,同时产生大量的IL2;再次用SPE刺激,可诱导T淋巴细胞凋亡。结论链球菌感染后,可能通过SPE诱导T淋巴细胞激活、随后出现细胞凋亡的途径而导致SLE病情活动加重。Objective To study the effect of proliferation and apoptosis of superantigen SPE in SLE.Methods To use SPE to stimulate T lymphocyte of SLE peripheral blood;To observe proliferation, cell phenotype and apoptosis on T lymphocyte with flow cytometry;to examine IL-2 production with ELISA.Results SPE have improved on proliferation of T lymphocyte of SLE peripheral blood,specially CD4^+ cell,and producing a large of IL-2.Again with SPE stimulation,induced T lymphocyte apoptosis.Conclusion After streptococcus infected,it induced T lymphocyte apoptosis activation through SPE and then appeared lymphocyte apoptosis to lead to aggravate the state of illness activation.

关 键 词：超抗原 红斑狼疮 系统性 T淋巴细胞 凋亡 增殖 

分 类 号：R446.6[医药卫生—诊断学]