平阳霉素白蛋白微球对血管内皮细胞形态的影响  

Effects of pingyangmycin albumin microspheres on the modality of blood vessle endothelial cells in vivo and in vitro

作　　者：郑根建[1] 高庆红[2] 周岚[1] 王晓毅[2] 温玉明[2] 王昌美[2] 

机构地区：[1]浙江省台州市中心医院口腔科,318000 [2]四川大学华西口腔医学院颌面外科

出　　处：《实用口腔医学杂志》2005年第4期511-514,共4页Journal of Practical Stomatology

基　　金：2002年浙江省台州市科委立项基金(022219);四川省科技攻关项目(03SG022-101)资助

摘　　要：目的:研究平阳霉素白蛋白微球(PYMAMS)对体外培养的人脐静脉血管内皮细胞株(ECV304)和兔耳中央动脉的血管内皮细胞形态的影响。方法:体外细胞实验中,通过倒置相差显微镜和电镜观察PYMAMS、PYM和AMS作用24、48、72、96h后的血管内皮细胞形态。动物实验采用区组随机法,于每只兔的双耳分别注射PYM水剂、PYM油剂、PYMAMS油剂。注射后2、7、14、21d,通过光镜和透射电镜观察兔耳中央动脉的组织学变化。结果:体外实验中,PYMAMS和PYM注射剂引起血管内皮细胞内出现空泡,细胞肿胀、变形、坏死。但AMS对血管内皮细胞没有损害。而动物实验中,PYM水剂组血管壁和血管内皮细胞无明显改变;PYM油剂注入后2、7d,血管内皮细胞肿胀,细胞内空泡,细胞脱落后有微血栓附着。21d有血管内膜及血管内皮细胞增生;PYMAMS组,注射后2、7d,血管内皮细胞出现肿胀、脱落,小血管远心端可见微球栓塞。注射后14d,血管内皮细胞增生,血管壁增厚,管腔缩小;微球有吸收改变。注射后21d,动脉管壁增生明显,个别细胞核呈分裂像,血管腔出现闭锁,小静脉内膜增生。结论:PYMAMS对血管内皮细胞造成的损伤较单一PYM明显。Objective: To evaluate the effect of pingyangmycin-albumin microspheres (PYM-AMS) on the modality of endothelial cells. Methods:In the in vitro study blood vessle endothelial cells of ECV 304 cell line were exposed to PYM-AMS(containing PYM at 150 μg/ml),AMS and PYM(150 μg/ml) for 24,48,72 and 96 h respectively. In the in vivo study,24 Japanese white rabbits were divided into 4 groups using randomized block design. PYM+9 g/L NaCl,PYM+soyabean oil, PYM-AMS+soyabean oil(PYM at 5 mg/ml) were injected into the central auricular arteries of the animals(0.26 ml/per ear), then these vessels were examined histologically 2, 7, 14, 21 days after injection respectively. The mophorlogy of the cells was observed by light microscope and electron microscope.Results:In vitro, a great part of cells were swollen and cell number decreased in PYM and PYM-AMS group. But there was no change in AMS group.In vivo, the endothelial cells had no significant changes in PYM+9 g/L NaCl group.In PYM+soyabean oil group, at the 2nd day, the endothelial cells were a little bit swollen. At the 7th day, some endothelial cells were dropped off. At the 21st day, a few of endothelial cells were proliferative. In PYM-AMS group, at the 2nd day, the endothelial cells were a little bit swollen and small vessels were embolized by PYM-AMS. At the 7th day, the endothelial cells were swollen. At the 14th day, the endothelial cells were proliferative and the wall of the central auricular artery had more layers. The lumen of the central artery became smaller, while the surface of PYM-AMS was absorbed. At the 21st day, the wall of the central auricular artery was proliferative and the artery became sclerostenosed. The PYM-AMS was obviously absorbed, while the wall of small vein was proliferative, too. Conclusion:PYM-AMS and PYM may injure blood vessel endothelial cells, the effect of PYM-AMS is more obvious than pingyangmycin.

关 键 词：平阳霉素 白蛋白微球 血管内皮细胞 细胞培养 血管 

分 类 号：R965[医药卫生—药理学]