含MT蛋奶粉对小鼠辐射损伤的保护作用  

Protective effects of egg-milk with MT on mice damaged by irradiation

作　　者：赵红光[1] 王志成[1] 杜翔[1] 吕喆[1] 姜晓艳[1] 史经宇 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]哈尔滨春源生物科技开发有限公司,黑龙江哈尔滨150036

出　　处：《吉林大学学报（医学版）》2005年第4期543-546,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(9630031)

摘　　要：目的:研究含金属硫蛋白(MT)蛋奶粉对小鼠电离辐射损伤的保护作用。方法:小鼠受2.5Gy(12.5mGy·min-1)X射线照射后,灌胃给予含MT蛋奶粉,连续14d后检测外周血白细胞数、淋巴细胞增殖率、股骨骨髓细胞DNA含量、胸腺细胞周期进程以及肝脏和肾脏丙二醛(MDA)含量和抗氧化酶(SOD、CAT和GSHPx)活性。结果:受照射小鼠给予含MT蛋奶粉后,尤其是含中高剂量MT蛋奶粉组,白细胞数、淋巴细胞增殖率和股骨骨髓细胞DNA含量明显高于辐射对照组(P<0.01);含MT蛋奶粉加照射组胸腺细胞G0/G1期细胞数明显低于辐射对照组(P<0.01),S期和G2/M期细胞数明显高于辐射对照组(P<0.01或P<0.05);照射组小鼠肝脏和肾脏MDA含量较正常对照组显著增加(P<0.01或P<0.05),抗氧化酶SOD、CAT和GSHPx活性则较正常对照组显著降低(P<0.01或P<0.05),而含MT蛋奶粉加照射组与辐射对照组比较,肝脏和肾脏MDA水平明显降低(P<0.01),抗氧化酶SOD、CAT和GSHPx活性显著增高(P<0.01或P<0.05)。结论:含MT蛋奶粉可明显改善X射线照射对小鼠的损伤效应,具有保护作用。Objective To explore the protective effects of egg-milk with metallothionein (MT) on mice damaged by irradiation. Methods After the mice were irradiated with 2.5 Gy (12.5 mGy·min^-1) X-rays, they were fed with egg-milk with MT for 14 days. The level of WBC in peripheral blood, lymphocyte proliferation rate, DNA content in marrow cells, malondiadehyde (MDA) levels and the activities of antioxidant enzymes (SOD, CAT and GSH-Px) in liver and kidney were measured. Results After the irradiated mice were fed with egg-milk with MT, especially in the egg-milk with MT of mild and high doses plus irradiation groups, the WBC number, lymphocyte proliferation rate and DNA content of marrow cells in the mice were significantly higher than those in the irradiation group (P<0.01), at the same time, the percentage of thymocyte G0/G1 phase of these mice were significantly lower than that of the irradiation group (P<0.01), and the percentage of thymocyte S and G2/M phase were significantly higher than those in the irradiation group (P<0.01 or P<0.05). Compared with the normal control, MDA levels in liver and kidney of the irradiation group increased significantly (P<0.01 or P<0.05), and the antioxidant enzyme activities of SOD, CAT and GSH-Px decreased significantly (P<0.01 or P<0.05). While in the egg-milk with MT plus irradiation groups, the MDA contents were significantly lower than those in the irradiation group (P<0.01) and the activities of SOD, CAT and GSH-Px were significantly higher than those in the irradiation group (P<0.01 or P<0.05). Conclusion The egg-milk with MT has protective effects on mice injuried by irradiation.

关 键 词：金属硫蛋白/药理学 辐射损伤 实验性 丙二醛 DNA 

分 类 号：R818.74[医药卫生—放射医学]