游离血红蛋白抑制淋巴细胞转化功能的研究  

Immunosuppressive effect of free hemoglobin on lymphocyte transformation function

作　　者：骆群[1] 张献清[2] 刘景汉[1] 

机构地区：[1]解放军总医院输血科,北京100853 [2]第四军医大学西京医院输血科,陕西西安710033

出　　处：《第四军医大学学报》2005年第14期1324-1325,共2页Journal of the Fourth Military Medical University

摘　　要：目的:研究游离血红蛋白(FHb)对淋巴细胞转化的抑制作用.方法:利用双向混合淋巴细胞反应(MLR)技术、细胞培养技术、及3H胸腺嘧啶(3HTdR)掺入法,将FHb按不同浓度比例加入MLR体系,及增加抗原提呈细胞(APC)浓度,以正常APC外周血单个核细胞(PBMC)的MLR为阴性对照,以环胞菌素A为阳性对照,计算上述因素对MLR的抑制率.结果:随着FHb浓度的增加,其抑制MLR的作用增强(P<0.01).两倍APC情况下可以明显减弱FHb对MLR的抑制作用(P<0.05).结论:FHb可通过干扰APC的提呈功能而抑制MLR,可以应用此方法来解释及证实大剂量输血致受者外周血的FHb增加从而对机体产生的免疫调节作用.AIM: To study the immunosuppressive effect of free hemoglobin on lymphocyte transformation function. METHODS: Using two-way mixed lymphocyte reaction (MLR), cell culture and incorporation of 3H-thymidine (3H-TdR), we added free hemoglobin (FHb) at different concentrations to the reaction system and raised the concentration of antigen presentation cells (APC) with normal APC concentration MLR as negative control and cyclosporin-A (CsA) as positive control. The ratio of the above factors in suppressing MLR was calculated. RESULTS: The suppressive effect of FHb increased with its concentration. When the concentration of APC in the system was twice that of the normal, the suppressive effect of free hemoglobin became weaker. CONCLUSION: Free hemoglobin may suppress MLR by disturbing the APC function. We can use this method to study the immunomodulation of large volume blood transfusion.

关 键 词：淋巴细胞培养试验 混合 血红蛋白类 抗原呈递细胞 

分 类 号：R457.1[医药卫生—治疗学]