重组人IL-11、G-CSF单独或联合应用对小鼠淋巴细胞免疫功能影响的比较研究  

Comparative study on the treatment effects with rhIL-11 and rhG-CSF in combination or alone on immune function

作　　者：赵杰[1] 赵翔宇[1] 黄晓军[1] 

机构地区：[1]北京大学人民医院血液病研究所,100044

出　　处：《中华血液学杂志》2005年第8期469-472,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目(30370591);教育部"211"工程资助项目;教育部博士点基金资助项目(20020001086);卫生部科研基金资助项目(200363)

摘　　要：目的探讨重组人(rh)IL-11、rhG-CSF联合应用对小鼠脾脏T淋巴细胞数量和功能的影响及其机制。方法应用流式细胞仪对细胞因子处理后的各组T淋巴细胞和亚群、共刺激分子CD28以及抑制性T淋巴细胞(CD3+CD4-CD8-、CD8+CD28-、CD4+CD25+)的数量进行测定;应用MTT法检测各组T淋巴细胞的增殖能力、混合淋巴细胞反应;测定细胞内IL-4、IFN-γ的分泌情况。结果经rhG-CSF单独及rhIL-11、rhG-CSF联合处理淋巴细胞总数和T细胞亚群与对照组比较均下降(P<0.05),而联合处理组CD3+、CD4+、CD8+细胞下降较明显,与其它各组比较差异有统计学意义(P<0.01),CD4+/CD8+细胞的比值联合处理组亦低于其它各组;G-CSF组及联合处理组CD3+CD28+细胞比例与对照组比较下降(P<0.01),CD4+CD28+、CD8+CD28+细胞联合处理组虽较其它各组低,但各组间差异无统计学意义;CD3+CD4-CD8-和CD4+CD25+抑制性T淋巴细胞各组间均无明显差异,而联合处理组CD8+CD28-抑制性T淋巴细胞较其它各组增加(P<0.05);细胞因子动员后各组T淋巴细胞增殖能力、供鼠对异种抗原的反应能力均降低(P<0.05),而联合处理组比其它各组下降更明显;联合处理组、G-CSF组与对照组比较细胞内细胞因子IFN-γ水平下降、IL-4水平升高,联合处理组与G-CSF组比较差异无统计学意义,但IFN-γ/IL-4的比值联合组低于其它各组(P<0.05)。结论rhIL-11与rhG-CSF体内应用后可产生协同作用,通过对T淋巴细胞数量和功能的影响来诱导机体免疫耐受。Objective To investigate the effect of in vivo administration of rhG-CSF and/or rhIL-11 on mice immune system function. Methods T cell subgroups, suppressor T cells (CD8^+CD28^-, CD4^+CD25^+, CD3^+CD4^-CD8^- T cells), expression of CD28 on T cells, and spleen T cells intracellur IL-4/IFN-γ secretion were determined by multicolor flow cytometry. MTT was used to determine the T cell proliferation capacity and mixed lymphocyte reactions.Results In vivo administration of cytokines decreased the percentage of lymphocytes (P<0.05), rhIL-11 and rhG-CSF in combination significantly decreased the CD4^(+)/CD8^(+) ratio and increased the percentage of CD8^(+)CD28^(-) suppressor T cells compared to either cytokine alone (P<0.01). There was no difference in the percentage of CD3^+CD4^-CD8^- and CD4^+CD25^+ suppressor T cells between either of the cytokines. Fourthermore, cytokines treatments significantly decreased the capacities of splenic T cells proliferation and the response to alloantigens compared with the PBS treatment (P<(0.05)), the combination group being more significantly decreased (P<0.01). And cytokines treatment significantly decreased the production of IFN-γ and increased the production of IL-4 compared with the PBS treatment(P<0.05).The ratio of IFN-γ/IL-4 were significantly decreased after the combination compared with either of them alone. Conclusion The combination of rhIL-11 and rhG-CSF is potentially synergistic in the induction of immune tolerance by their effects on the proliferation capacity and function of T lymphocytes.

关 键 词：重组人IL-11 G-CSF 淋巴细胞 免疫功能 比较研究 T淋巴细胞 

分 类 号：R96[医药卫生—药理学]