随机引物PCR法基因分型肺炎克雷伯菌  

Genotyping of Kelbsiella Pneumoniae by Arbitrarily Primed Polymerase Chain Reaction Technique

作　　者：叶明亮[1] 刁亚林[1] 侯晓琦[1] 

机构地区：[1]济南军区总医院输血科,山东济南250031

出　　处：《中国误诊学杂志》2005年第8期1403-1405,共3页Chinese Journal of Misdiagnostics

基　　金：全军医药卫生基金项目(编号:98Q012)

摘　　要：目的:对肺炎克雷伯菌进行随机引物PCR(AP-PCR)法基因分型。方法:以单一随机引物及优化的反应体系对临床分离的199株肺炎克雷伯菌(K.pneumoniae)进行AP-PCR分析并按指纹图上DNA条带数及片段大小绘制基因分型图谱。结果:199株临床分离K.pneumoniae共得158种AP-PCR型。结论:AP-PCR法可将K.pneumoniae分型158种。Objective:To type Klebsiella pneumoniae(K.pneumoniae) by arbitrarily primed polymerase chain reaction(AP-PCR)technique.Methods:The single random primer and optimal reaction system were used for genotyping of the 199 strains of K.pneumoniae isolated from clinical samples, and the fingerprinting profiles were drawn according to the number and length of DNA bands.Results:158 kinds of AP-PCR types were obtained in the 199 strains of K.pneumoniae isolated from clinical samples.Conclusion:158 kinds of genotype profiles could be obtained by AP-PCR technique.

关 键 词：克雷伯菌 肺炎/遗传学 聚合酶链反应 基因型 

分 类 号：R378.23[医药卫生—病原生物学]