豚鼠心内注射给药后外耳淋巴液和血浆地塞米松磷酸钠的浓度测定  

Determination of the Content of Dexamethasone Sodium Phosphate in the Scala Tympani Perilymph and Blood Plasma in Guinea Pigs after Intracardiac Injection of the Drug

作　　者：张顺国[1] 陈敏玲[1] 李方[1] 杨军[2] 侯东明[2] 吴皓[2] 

机构地区：[1]上海第二医科大学附属新华医院药剂科,200092 [2]上海第二医科大学附属新华医院耳鼻咽喉-头颈外科,200092

出　　处：《医药导报》2005年第8期662-664,共3页Herald of Medicine

基　　金：上海市科委科研计划项目(基金编号:0341-19819)

摘　　要：目的建立测定豚鼠外耳淋巴液和血浆中地塞米松磷酸钠含量的方法。方法采用高效液相色谱法测定淋巴液和血浆地塞米松磷酸钠的浓度。固定相:ZORBAXEclipseXDB-C18色谱柱(4.6mm×250mm,5μm);流动相:7.56mmol·L-1硫酸铵-乙腈(70∶30);流速:1.0mL·min-1;柱温:20℃;检测波长:239nm。结果地塞米松磷酸钠浓度测定的线性范围为0.05~4.80mg·L-1,r=0.9998,最低检测限为6.25μg·L-1,日内和日间RSD分别为1.7%和2.3%。给药后1h鼓阶外淋巴液中地塞米松磷酸钠的浓度为0.08mg·L-1,血浆中地塞米松磷酸钠浓度为2.77mg·L-1。结论该方法灵敏、准确、重现性好,可用于血浆和外耳淋巴液中地塞米松磷酸钠的浓度测定。全身给药治疗内耳疾病不是适宜的给药方式,应该探索其他给药途径。Objective To set up a method for the determination of the concentration of dexamethasone sodium phosphate in perilymph and blood plasma of guinea pigs. Methods HPLC was used for the determination of the content of dexamethasone sodium phosphate in the samples of perilymph and blood plasma after intracardiac injection of the drug .HPLC conditions: A ZORBAX Eclipse XDB-C_18 column (4.6 mm×250 mm,5 μm)served as the stationary phase,the mobile phase was 7.56 mmol·L^-1 ammonium sulfate- acetonitrile (70∶30) , with a flow rate of 1.0 mL·min^-1; the column temperature was 20℃, the wavelength for detection, 239 nm. Results The calibration curve of dexamethasone sodium phosphate was linear within the concentration range of 0.05-4.80 mg·L^-1(r=0.999 8), the lower limit of detection was 6.25 μg·L^-1, the intra-day and inter-days RSD were 1.7% and 2.3%. One hour after the administration of the drug, the concentrations of dexamethasone sodium phosphate were 0.08 mg·L^-1 and 2.77 mg·L^-1 in the perilymph and blood plasma ,respectively. Conclusion The method was shown to be sensitive, accurate and well reproducible ,it can thus be used for the determination of the concentration of dexamethasone sodium phosphate in perilymph and blood plasma. Our results also suggest that systemic medication of the drug is not a suitable method for the treatment of diseases of the inner ear. Other routes for the administration of the drug, therefore, should be sought.

关 键 词：地塞米松 磷酸钠 淋巴液 血浆 高效液相色谱法 含量测定 

分 类 号：R977.1[医药卫生—药品] R965.2[医药卫生—药学]