补肾壮阳方对更年期肾阳虚证大鼠的影响  

The Effect of Bushenzhuangyang Decoction on Climacteric Rats with Insufficiency of Kidney-Yang

作　　者：司卉良[1] 陈友香[1] 侯安继[1] 谢丹[1] 

机构地区：[1]武汉大学中南医院中医科,430071

出　　处：《医药导报》2005年第8期675-677,共3页Herald of Medicine

摘　　要：目的研究补肾壮阳方对更年期肾阳虚证模型大鼠的治疗作用。方法30只11~15个月龄自然更年期雌性SD大鼠,随机平均分为模型组、治疗组和对照组,10只3~5个月龄雌性SD大鼠设为青年组。模型组和治疗组肾阳虚证模型制作成功后,治疗组给予补肾壮阳方煎剂灌胃,共14d。实验第29天,测定各组大鼠雌二醇(E2)、促卵泡激素(FSH)、黄体生成素(LH)水平。结果与模型组比较,治疗组大鼠脱毛较少,食欲增强,多尿、畏寒状况改善,体重明显增加(P<0.01);治疗组大鼠血中E2明显高于对照组(P<0.01),LH、FSH低于模型组(P<0.05)。结论补肾壮阳方对更年期肾阳虚证模型大鼠有治疗作用。Objective To study the therapeutic effect of bushenzhuangyang(BSZY) decoction on the model of female climacteric rats with insufficiency of kidney-yang. Methods 30 female SD rats at their natural climacteric age of 11-15 months were randomly divided into 3 equal groups:1.model(M) group,2. M+BSZY treatment group and 3.Climacterium group.The model of insufficiency of kideney-yang was set up by giving an I.M. injection of 2 mg·(100 mg)^-1 of cortisol to each rat at 8 a.m. q.d. for 14 consecutive days. In addition, 10 female SD rats at the age of 3-5 months served as young controls. Beginning from day 15,that is ,1 day after the model had been successfully set up ,rats of group 2 were given each by gastrogavage 10 mL·kg^-1 of BSZY decoction at 9 a.m.q.d.for 14 consecutive days .Rats of group 1,2 and young controls were given each equivalent amounts of 0.9%sodium chloride solution by the same route for 14 days as well. On day 29, blood samples were collected from rats of all 4 groups for the determination of serum levels of estradiol (E_2) , follicle-stimulating hormone(FSH) and luteinizing hormone(LH). Results In comparison with rats of the model group (group 1) , rats of group 2 treated with BSZY had a depilation of milder degree , better appetite,amelioration of polyuria and chilly sensation. as well as a striking increase in body weight(P<0.01).The serum level of E_2 in rats treated with BSZY was significantly higher than that of the model group(P<0.01),while the levels of serum FSH and LH in rats treated with BSZY were apparently lower than those in rats of the model group (P<0.01 or P<0.05).The serum level of E_2 in rats of the model group was much lower than that of rats in the climacterium (group 3) (P<0.01). Conclusion BSZY was shown to be effective in the treatment of insufficiency of kidney-yang in climacteric rats.

关 键 词：补肾壮阳方 更年期综合征 肾阳虚证 大鼠 

分 类 号：R282[医药卫生—中药学] R256[医药卫生—中医学]