SARS冠状病毒刺突蛋白在裂殖酵母中的表达  

Expression of SARS Spike Gene in Schizomycete pombe

作　　者：吴朝霞[1] 郑文岭[1] 张宝[2] 师永霞[2] 马文丽[2] 

机构地区：[1]华南理工大学生物科学与工程学院 [2]华南基因组研究中心,广州510860

出　　处：《生物工程学报》2005年第4期638-641,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助项目(No.3988044);广州市重大科技项目(No.1999-Z005-001)~~

摘　　要：根据SARS冠状病毒S蛋白主要抗原表位的分析,将S蛋白基因扩增成大小为800bp^1000bp的5个片段,分别与裂殖酵母载体pNMT1连接,经电穿孔转化TCP1菌株,得到诱导表达5个片段的裂殖酵母菌株。对诱导表达产物进行SDS-PAGE和Westernblot分析表明,S基因5个片段在裂殖酵母中分别得到不同程度的表达,为进一步研究新一代SARS疫苗提供了材料。The viral spike protein is the main surface antigen of the coronavirus, and it could be useful in the research of clinical diagnosis, SARS vaccine and the structure biology.According to the analysis of the main antigen of the SARS spike protein, 5 fragments of the whole spike gene were cloned,and ligated to the vector pNMT1. Through electroporation transformantion to TCP1, the recombinant S.pombe strains capable of expressing the 5 fragments were constructed. SDS-PAGE or Western blot analysis of the induced expression products demonstrated that the 5 recombinant proteins were expressed in the fission yeast respectively.

关 键 词：SARS 刺突蛋白 裂殖酵母 表达 

分 类 号：R392[医药卫生—免疫学]