NRSE与NRSF及其对神经元特异性基因表达的调控作用  

NRSE,NRSF and Their Modulatory Effects on The Expression of Neuronal-specific Genes

作　　者：王小飞[1] 于盼盼[1] 陆佩华[1] 

机构地区：[1]上海第二医科大学神经生物学实验室

出　　处：《生物化学与生物物理进展》2005年第7期595-599,共5页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划项目(973)(2003CB515302);上海市科技发展基金资助项目(02JC14014)~~

摘　　要：神经限制性沉默元件(NRSE)是一段长度为21￣23bp的保守DNA序列,存在于许多神经元特异表达基因的转录调控区中,神经限制性沉默因子(NRSF)能特异性结合到NRSEdsDNA上,并通过其N端和C端阻遏结构域分别连接共阻遏蛋白Sin3A/B和CoREST,Sin3A招募HDAC对组蛋白进行去乙酰基化修饰,CoREST则作为平台蛋白招募特异的“沉默组件”,以此维持基因沉默.最近的研究显示,NRSEdsRNA能在转录水平与NRSF蛋白直接作用,而不是作为siRNA或miRNA在转录后水平启动神经元特异性基因的表达.Neuron-restrictive silencer element or repressor element-1 (NRSE/RE1), present in the transcriptional regulatory regions of multiple neuronal-specific genes, is a 21 similar to 23 bp conservative DNA sequence. Neuronal restricted silencing factor or RE1-silencing transcription factor (NRSF/REST) can bind to the NRSE, and then the gene repression was mediated in part through the association of its NH2-terminal repression domain with the corepressor mSin3, resulting in the recruitment of histone deacetylase (HDAC) and consequent acetylization, and its COOH-terminal repression domain with the corepressor CoREST, that may serve as a platform protein for assembly of specialized repressor machinery. The recent study show that NRSE dsRNA can trigger gene expression of neuron-specific genes through interaction with protein NRSF at transcriptional level, rather than through siRNA or miRNA at posttranscriptional level.

关 键 词：神经元 调控作用 基因表达 NRSF 特异表达基因 DNA序列 转录调控区 特异性结合 dsRNA 特异性基因 转录后水平 miRNA siRNA 阻遏蛋白 基因沉默 直接作用 转录水平 限制性 结构域 酰基化 组蛋白 F蛋白 C端 N端 

分 类 号：Q78[生物学—分子生物学] TP18[自动化与计算机技术—控制理论与控制工程]