黄芪总黄酮对γ射线所致V79细胞DNA链断裂损伤防护作用的实验研究  

Protective effect of total flavonoids of astragalus mongholicus Bunge on ONA strand breaks,in V79 cells caused by γ-rays

作　　者：汪德清[1] 沈文梅[1] 田亚平[1] 刘国廉[1] 杨素霞[1] 周淑珍[1] 孙仲怡[1] 

机构地区：[1]解放军总医院生化科,北京放射医学研究所

出　　处：《中华放射医学与防护杂志》1995年第2期106-107,共2页Chinese Journal of Radiological Medicine and Protection

摘　　要：本文作者用ＤＮＡ解旋的荧光检测（ＦＡＤＵ）方法观察了γ射线对Ｖ７９细胞ＤＮＡ的损伤作用及黄芪总黄酮对其作用的防护效果。结果表明细胞ＤＮＡ链断裂率与γ射线的照射量成正比。黄芪总黄酮浓度为０．６ｍｇ／ｍｌ时即对ＤＮＡ损伤有保护作用，当浓度达１．２ｍｇ／ｍｌ时，对γ射线所致ＤＮＡ链断裂损伤有非常显著的防护效果。In the report DNA strand breaks in V79 cells caused by γ-rays and the protective effect of total flavonoids of astragalus mongholicus Bunge(TFA)have been studied by fluorometric assay of DNA unwinding.The results indicated that the rate of DNA strand break increased with the increase of γ-ray doses.Even When the concentration of TFA was as low as 0.6/ml,it still showed some protective effect on DNA strand breaks.When its concentration was up to l.2mg/ml,TFA could effectively protect DNA from radiation damage.

关 键 词：Γ射线 V79细胞 DNA 黄芪总黄酮 荧光检测 

分 类 号：R818.023[医药卫生—放射医学]