北京荷斯坦母牛7个微卫星基因座遗传多态性研究  

Genetic Polymorphisms of Seven Microsatellite Loci in Beijing Holstein Cows

作　　者：周国利[1] 储明星[2] 金海国[3] 石万海[4] 曹福存[4] 朱颜[1] 

机构地区：[1]聊城大学生物系,聊城252059 [2]中国农业科学院畜牧研究所,北京100094 [3]延边大学农学院动物科学系,龙井133400 [4]北京奶牛中心,北京100085

出　　处：《安徽农业大学学报》2005年第3期293-297,共5页Journal of Anhui Agricultural University

基　　金：北京市自然科学基金项目(6002014)资助。

摘　　要：利用7个微卫星基因座BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21对240头北京荷斯坦母牛进行遗传检测,用非变性(中性)聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物。结果表明BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21的等位基因数/多态信息含量/有效等位基因数/杂合度分别为4/0.3869/1.7693/0.4348、5/0.5923/2.9121/0.6566、8/0.7114/3.9012/0.7437、6/0.5921/2.8244/0.6459、6/0.7122/3.9989/0.7499、6/0.7144/4.0124/0.7508和7/0.6154/2.9805/0.6645。可见BM4505的遗传变异最大,BM1818的遗传变异最小。The polymorphisms of seven microsatellite loci BM1818, BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were detected in 240 Beijing Holstein cows.The PCR amplified products of microsatellites were detected by non-denatured (neutral) polyacrylamide gel electrophoresis. The number of alleles/polymorphic information content/ effective numbers of alleles/heterozygosity of BM1818,BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were 4/0.3869/1.7693/0.4348, 5/0.5923/2.9121/0.6566, 8/0.7114/3.9012/0.7437, 6/0.5921/2.8244/0.6459, 6/0.7122/3.9989/0.7499, 6/0.7144/4.0124/0.7508 and 7/0.6154/2.9805/0.6645, respectively. The results revealed the greatest genetic variation in BM4505 and the lowest in BM1818.

关 键 词：北京荷斯坦母牛 微卫星基因座 遗传多态性 

分 类 号：S823.912[农业科学—畜牧学]