成纤维细胞生长因子增强骨形态发生蛋白在小鼠体内骨诱导作用  

FIBROBLAST GROWTH FACTOR ENHANCES BONE FORMATION INDUCED BY BONE MORPHOGENETICPROTEIN IN MICE

作　　者：李亚非[1] 胡蕴玉[1] 吕荣[1] 宋海燕[1] 

机构地区：[1]第四军医大学西京医院骨科研究所

出　　处：《中华骨科杂志》1995年第12期837-838,共2页Chinese Journal of Orthopaedics

摘　　要：在啮类动物体内植入骨形态发生蛋白（ＢＭＰ）后，引起细胞内一系列生化和形态学变化，在植入物内和其周围形成新骨。这过程受多因素影响。本实验将重组人成纤维细胞生长因子（ｂＦＧＦ）与部分纯化的牛ＢＭＰ悬液混合后注入小鼠肌肉，观察成骨反应。单独注射牛骨ＢＭＰ组和ｂＦＧＦ组为对照。２１天时在ｂＢＭＰ／ｂＦＧＦ组及单独ｂＢＭＰ组均有新骨形成，但ｂＢＭＰ／ｂＦＧＦ组的钙量是单独ｂＢＭＰ组的３倍（Ｐ值＜０．０１）并有较明显的血管增生现象。Abstract Implantation of bone momorphogenetic protein(BMP) in rodents elicits a series of cellular events leading to the formation of new boneinside and adjacent to the im plants. It hasbeen suggested that local growth factors mayfurther regulate tke process once it has beeninitlated. The experiment was designed tostudy the effect of adding a growth factor tothe inductlve implant. 100ng of recombinantkuman basic fibroblast growth factor (bFGF) was added to tke suspension containing 4mg ofpartially purifled hBMP(group A) which wasinjected into muscle of mice. The other im-plants served as controls.They contained ei-ther 4mg of bBMP without BFGF(group B) or100ng of bFGF alone(group C).Bone forma-tion was induced in group A and B after 3weeks.The amount of mineralized tissue inthe implants of group A was 3 times greate rthan in tke group B(P<0.01).

关 键 词：成纤维细胞 生长因子 骨诱导 骨形态发生蛋白 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]