人血清TRH放射免疫分析及临床应用研究  

RADIOIMMNOASSAY OF THYROTROPIN RELEASING HORMONE IN HUMAN SERUM AND ITS CLINICAL APPLICATION

作　　者：赵志英[1] 刘世贞[1] 

机构地区：[1]北京协和医院核医学科

出　　处：《中华核医学杂志》1995年第3期154-157,共4页Chinese Journal of Nuclear Medicine

摘　　要：探讨外周血中促甲状腺激素释放激素（ＴＲＨ）浓度与下丘脑-垂体-甲状腺轴功能生理、病理变化的关系，尤其是各种甲状腺疾病时血中ＴＲＨ浓度变化的特点及临床意义．方法：采用自制的抗ＴＲＨ抗血清及１２５Ｉ-ＴＲＨ，建立了高度灵敏、特异的血清ＴＲＨＲＩＡ直接测定法．血样品采集后用硫酸８-羟基喹琳、吐温-２０和ＥＤＴＡ混合物抗灭活．结果：方法灵敏度为８．３ｐｍｏｌ／Ｌ，批内及批间ＣＶ分别为２．５％～４．９％和８．９％～１０．０％，回收率为９２％～１０５％．测定４０４例正常人及垂体、甲状腺疾病患者的血清ＴＲＨ值，甲亢患者显著下降，原发性甲低患者明显升高，结节性甲状腺肿、冷结节、生长激素缺乏症及垂体瘤患者血清ＴＲＨ值与正常人差异无显著性．结论：血清ＴＲＨ浓度的变化与甲状腺功能状态密切相关，外周血ＴＲＨ水平的测定对研究下丘脑-垂体-甲状腺轴功能生理、病理状态有重要意义．PURPOSE The study was aimed at investigating the relationship between the TRH level in peripheral blood and the physiological, pathophysiological variation of the hypothalamic-pituitary-thyroid axis, especially at studying the rela tionship between TRH level and various thyroid function states as well as its clinical significance. METHODS A highly sensitive, specific radioimmunoassay of the thyrotropin releasing hormone (TRH) has been developed in our laboratory using 125I labeled anti-TRH antibodies. Blood samples were collected in tubes containing a mixture of 8-hydroxyquinoline sulfur, Tween 20 and EDTA-2NA(E). RESULTS The sensitivity was 8. 3pmol / L, and the intra and inter-assay coefficients of variation were 2.5%～4.9% and 8. 9% ～10.0% respectively. The recovery rate ranged from 92% ～ 105%. The TRH levels in sera of 80 normals and 324 patients with pituitary thytoid diseases were measured with this assay. The results show a significant decrease of TRH levels in hyperthyroid patients and increase in hypothyroid subjects. The TRH levels in patients with nodular goiter and cold nodules, pituitary dwarfism and pituitary tumours were not significantly different from those of the normal group. CONCLUSIONS The results demonstrated that measurement of TRH level in Peripheral blood is useful in investigating the physiological and pathophysiological status of the hypothalamic- pituitary-thyroid axis function and the change in TRH levels has close relations with th thyroid function.

关 键 词：甲状腺疾病 血清 TRH 放射免疫测定 临床应用 

分 类 号：R581.04[医药卫生—内分泌]