高效液相色谱法测定苦参素胶囊中氧化苦参碱及有关物质的含量  

Determination of Oxymatrine Content and Its Related Substances in Oxymatrine Capsule by HPLC

作　　者：邱文炜[1] 简淑娟[1] 龚惠伉[1] 

机构地区：[1]广东省惠州市药品检验所,广东惠州516008

出　　处：《中药材》2005年第7期601-603,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:测定苦参素胶囊中氧化苦参碱的含量与氧化槐果碱等有关物质的量.方法:采用HPLC法,色谱柱为C18柱,流动相为磷酸盐缓冲液-甲醇(85:15),检测波长为210 nm.结果:氧化苦参碱线性范围为6.952～20.856μg/ml(r=0.9999),氧化槐果碱线性范围为0.1837～0.5510μg/ml(r=0.9997).结论:所用方法简便、快速、准确、专属性好.Objective: To determine oxymatrine content and its related substances in oxymatrine capsule. Methods: A HPLC method was adopted. C_ 18 column was used. The mobile phase consisted of phosphate buffer solution-methanol(85∶15). The UV detective wavelength was 210 nm. Results: The calibration curve of oxymatrine was linear in the range of 6.952～20.856 μg/ml (r=0.9999). The calibration curve of N-oxysophocarpine was linear in the range of 0.1837～0.5510 μg/ml(r=0.9997). Conclusions: The method is simple, rapid and accurate.

关 键 词：氧化苦参碱 苦参素胶囊 有关物质 高效液相色谱法 含量 测定 氧化槐果碱 磷酸盐缓冲液 线性范围 HPLC法 C18柱 检测波长 色谱柱 流动相 专属性 

分 类 号：R512.62[医药卫生—内科学] R259.126.2[医药卫生—临床医学]