卡介苗刺激的肺泡巨噬细胞上清液对肺成纤维细胞的作用  

The enhancing effects of the supernstant of pulmonaryalveolar macrophages stimulated by BCG on prolifera-tion of pulmonary fibroblasts.

作　　者：张尚明[1] 徐国仓 罗玲[1] 户万秘[1] 曹兆丰[1] 王秋菊[1] 

机构地区：[1]华北煤炭医学院病理研究室,华北煤炭医学院微生物教研室

出　　处：《中华结核和呼吸杂志》1995年第1期26-28,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：煤炭部科学研究基金

摘　　要：７．５～６０ｍｇ／Ｌ卡介苗（ＢＣＧ）刺激的肺泡巨噬细胞（ＰＡＭ）培养上清液能显著促进肺成纤维细胞（ＰＦＢ）增殖，发现该上清液中含较高水平的肿瘤坏死因子（ＴＮＦ），且其促ＰＦＢ增殖作用与ＴＮＦ水平成正相关（ｒ＝０．９７），提示ＢＣＧ在体外能刺激ＰＡＭ产生ＴＮＦ，ＴＮＦ为该上清液促ＰＦＢ增殖的主要因素。５０ｍｇ／ＬＳｉＯ＿２刺激的ＰＡＭ上清液亦能促进ＰＦＢ增殖，但上清液中几乎检测不到ＴＮＦ，说明其促ＰＦＢ增殖的成份是不同于ＴＮＦ的其它物质，ＴＮＦ能协同该物质促ＰＦＢ增殖。上述结果提示结核菌刺激ＰＡＭ产生ＴＮＦ可能是矽肺结核病较单纯矽肺发展迅速的一个重要原因。he supernatant of pulmonary alveolarmacrophages (PAM) stimulated by 7. 5 ～ 60mg/L ofBCG could significantly enhance proliferation of pul-monary fibroblasts (PFB) , and the supernatant con-tained high level of TNF. This result indicated BCGcould stimulate PAM to produce TNF in vitro. On theother hand, the supernatant of PAM stimulated by50mg/L of SiO_2 could also enhance proliferation of PFB ,but the level of TNF in the supernatant was very lowand it demonstrated the ingredient in the supernatantenhancing proliferation was not TNF. TNF showedsynergisic effect with this ingredient for enhancing pro-liferation of PFB. Therefore, TNF produced by tuber-culosis bacteria-stimulated PAM might be an importantfactor enhancing the devefopment of fibrosis of silicotu-bercufosis.

关 键 词：卡介苗 矽肺 结核病 肺泡巨噬细胞 鼠 动物实验 

分 类 号：R135.2[医药卫生—劳动卫生]