以嗜碱性粒细胞脱颗粒实验研究在真菌过敏性哮喘患者中的应用  

Application of human basophil degranulation test(HBDT)in fungal allergic asthmatic patients.

作　　者：顾明亮[1] 张学智[1] 

机构地区：[1]山东省东营胜利石油管理局中心医院

出　　处：《中华结核和呼吸杂志》1995年第3期158-160,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：从嗜碱粒细胞水平探讨研究真菌过敏原的方法学，并且应用于过敏性哮喘的研究。实验显示：在ｐＨ７．６、３７℃、真菌抗原浓度１０￣（－１）ｍｇ／ｍｌ的条件下，应用人嗜碱粒细胞脱颗粒实验（ＨＢＤＴ）研究真菌过敏原的方法具有很高的特异性（ｔ＝１６．８，Ｐ＜０．００１）和重复性（ＣＶ：８．７％）。应用ＨＢＤＴ对真菌过敏性哮喘进行研究，发现ＨＢＤＴ与皮肤试验的结果具有很高的符合率（９１．７％）。过敏性哮喘的脱颗粒指数（ＤＩ）显著高于正常人，并且脱敏治疗１年能显著降低其Ｄ１。提示：ＨＢＤＴ是一种简便、准确、可靠的体外研究过敏原的方法，反映了嗜碱粒细胞的功能状况。he authors recommand the methdology of HBDTfor testing fungal allergens,pH 7.6,37 ℃ and 10mg/ml are the most suitable experimental conditions.The positive rate of HBDT in allergic asthmatic patientsis 91.7%,and correlated very well with the results ofskin tests. The degranulation index(DI)in allergicasthma is significantly higher than that in normals(P<0.001).The DI after one year's desensitization treat-ment with fungal allergens is markedly lowered than be-fore(P<0.001).The results suggest that HBDT is asimple,reliable and precise method to investigate aller-gens in vitro and also reflects the function of basophils inasthma.

关 键 词：嗜碱细胞 真菌 过敏 哮喘 病理 

分 类 号：R562.250.2[医药卫生—呼吸系统]