低氧致大鼠肺中血小板－衍化生长因子－β受体基因表达增强  

Increased expression of PDGF-βreceptor gene in hy-poxic rats lungs

作　　者：鲁青 孙仁宇[1] 余漫 张宏[1] 薛全福[1] 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《中华结核和呼吸杂志》1995年第4期207-208,共2页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家"八五"攻关资助课题

摘　　要：观察血小板。衍化生长因子－β（ＰＤＧＦ－β）受体在低氧大鼠肺中基因表达的变化。Ｎｏｒｔｈｅｒｎ杂交显示正常大鼠肺中可表达ＰＤＧＦ－β受体的信使ＲＮＡ（ｍＲＮＡ）。随低氧时间的延长ｍＲＮＡ水平迅速增加，至低氧第４日时达高峰，为正常的１．３倍（Ｐ＜０．０５）。免疫组化显示ＰＤＧＦ－β受体主要分布在肺的中、小动脉的平滑肌细胞及内皮细胞。随低氧时间的延长，其分布无变化，但密度显著增加，至低氧第７日时达高峰，为正常的２．４倍（Ｐ＜０．０５）。提示ＰＤＧＦ－β受体基因表达增强在低氧性肺血管重建中起一定作用。he changes of platelet-derived growth factor(PDGF)βreceptor gene expression in hypoxic ratslungs was examined. Northern blots analvsis revealedthat normal lungs expression PDGF-β receptor mRNA,with the longer of hypoxia the level of the mRNAincreased rapidly. It reached a maximum at day 4,andwas 1.34 fold as compared with the control(P<0.05)。Immunohistochemistry investigation showed thatPDGF-β receptor mainly distributed on smooth musclecells and endothelial cells of middle and small arterialtrees in rat lung. With hypoxia,the distribution ofPDGF-β receptors did not change,but it was moreintense and reached a maximum at day 7, and was 2.40fold as compared with the control(P< 0.05).The re-sults suggested t hat inc rea sed ex pression of PDGF re-ceptor gene may play a role in hypoxic pulmonary vascu-lar remodeling.

关 键 词：缺氧血症 肺动脉高压 血小板 衍化生长因子 大鼠 

分 类 号：R543.202[医药卫生—心血管疾病] R594.302[医药卫生—内科学]