转化生长因子－β对人胚肺成纤维细胞胶原表达的影响  

The effect of transforming growtk factor-β on collagenexpression hy human embryonic fibroblasts.

作　　者：刘新民[1] 何冰[1] 宿利 赵桂荣[1] 

机构地区：[1]北京医科大学第一临床学院

出　　处：《中华结核和呼吸杂志》1995年第5期287-289,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金

摘　　要：转化生长因子－β（ＴＧＦ－β）是一族多功能的细胞因子，具有调节细胞外基质代谢的功能，在肺间质纤维化的形成中具有重要作用。胚原是细胞外基质的主要成分。胶原积聚是肺间质纤维化的一个重要特征。为了进一步探讨肺间质纤维化形成的机制，在体外观察了ＴＧＦ－β对人胚胎肺成纤维细胞胶原蛋白合成及胶原Ｉ、胶原ＩＩｌｍＲＮＡ表达的影响。结果发现：ＴＧＦ－β能刺激胶原蛋白的合成，胶原Ｉ、胶原ＩＩＩｍＲＮＡ的表达。而对成纤维细胞的增殖影响不明显。因此认为ＴＧＦ－β可能与肺间质纤维化时胶原积聚有关。ＴＧＦ－β胶原合成的调节是通过ｍＲＮＡ水平。ransforming growth factor-β (TGFS) is a cy-iokine with diverse biological activity. It can regulatethe metabolic function of extracellular matrix (ECM)and play an important role in the development of pul-monary fibrosis. Collagen are major ECM componentsthat are responsible for normal lung structure and func-tion. Collagen accumulation is a major feature of pul-monary fibrosis. In order to study the mechanism ofpulmonary fibrosis, we examined the effects of TGF-βon the production .mRNA expresstion of type 1 collagenand type Ⅳ collagen by cultures of human embryoniclung fibroblasts. The resulis indicated that TGF-β caninduce collagen forrnation and type Ⅰcollagen, type Ⅳcollagen mRNA exprcssion without affecting cell Prolif-eration. We concluded that TGF-βplays a role in colla-gen accumulation of pulmonary fibrosis and the changesin collagen production appear to be mediated on collagenmRNA level.

关 键 词：肺纤维变性 转化生长因子 成纤维细胞 胶原 

分 类 号：R563.130.2[医药卫生—呼吸系统]