结核杆菌菌尘气溶胶感染豚鼠的实验研究  

Guiuea pigs infected by bacterial dust aerosol of Bacil-Ius tuberculosis

作　　者：吴兆儒[1] 王忠仁[1] 宛淑贤 童小玫 

机构地区：[1]北京市结核病胸部肿瘤研究所流行病学研究室

出　　处：《中华结核和呼吸杂志》1995年第5期313-315,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：结核杆菌菌尘埃传播至今仍然是一个有争议的问题：为此我们将结核杆菌与干燥的尘土混合，用气溶胶发生器喷洒，豚鼠头暴露。尘土中４μｍ以下粒子占５．１％，菌尘气溶胶中细菌浓度为３８６．７ｃｆｕ／Ｌ其质量中值直径（ＭＭＤ）为１．９μｍ。豚鼠暴露后７０天，暴露３分钟组７只豚鼠中有２只结核杆菌素阳转，１只的肺和脾脏分离培养到结核杆菌；暴露１８分钟组８只豚鼠中有５只结核杆菌素阳转，其中１只的脾脏，２只的肺脏培养到结核杆菌。结果表明，结核杆菌可以通过菌尘气溶胶传播。here stili is controversy over whether Bacillus tu-berculosis could be spread by dust. For the purpose to il-lustrate the possible contagious rouie. guinea pigs wereexposed to bacterial dust aerosol which was generated bymixing Bacillus tuberculosis H37Rv with dried dust.The percentage of the size of particles, diameter<4μm. was 5. 1%. The density of bacteria in the dustaerosol was 386. 7 cfu/L and the mass mediurn diameter(MMD) was 1. 9 Pm. On the 70 days after exposure . inthe 3 minutes exposed group. positive reaction to OTtest (1 : 20) was 2/7, culture of Bacillus tuberculosiswas positive from lungs and spleeD was 1/7, in the 18minutes exposed group. 5/8 were positive for OT test ,the positive culture found from lungs was 2/8 and fromspleen 1/8. These results showed that Bacillus tubercu-losis could be spread by bacterial aerosol.

关 键 词：结核杆菌感染 气溶胶 感染 豚鼠 病菌传播 

分 类 号：R378.911[医药卫生—病原生物学]