肺心病患者绿脓杆菌分型耐药质粒DNA分子杂交的临床意义  

The clinical significance of Pseudomonas aeruginosa typing and R-plasmid and DNA molecule hybridization in patients with cor pulmonale

作　　者：缪竞智[1] 张秀珍[1] 李焕娄[1] 何依萍 王小平[1] 张桂兰[1] 林凤如[1] 

机构地区：[1]卫生部北京医院,中国医学科学院医药生物技术研究所

出　　处：《中华结核和呼吸杂志》1995年第6期357-359,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：对８所医院住院肺心病患者痰培养获绿脓杆菌（ＰＡ）６００株进行分型分析，结果Ｐ６型占２５．６％。不同地区和医院的假单胞菌及ＰＡ型别分布不同，可作为流行病学调查依据。１５０株中的６８株ＰＡ含有质粒，用β－内酰胺酶和氨基苷钝化酶耐药基因探针各６种，与６０株含质粒的ＰＡ菌落杂交，证实从院内感染患者分离到的３株ＰＡ均带有与ＯＸＡ－２、ＡＡＣ（６＇）－１ｂ同源的ＤＮＡ序列并均来自同一个菌株，在传播院内感染时，又获得了新的耐药基因。00 strains of Pa were typed from the sputa of pa-tients with cor pulmonale in 8 hospitals. P6 type stoodsfirst, accounting for 25.6%,and the types of PA andthe difference in distribution of Pseudomonas from dif- ferent districts and hospitals, might be used as an im- portant parameter in the epidemiological study.68 of150 strains from patients with cor pulmonale containedplasmids. 60 isolates harbOring plasmids colonies werehybridized with 6 β-lactamase and 6 aminoglycosidemodifying enzyme resistance gene probes, The experi- ments demonstrated the 3 strains isolated from patientswith nosocomial infection came from one same strainand got a new resistance gene during spreading of noso-comial infection, and these isolates contained homolo- gous sequence with OXA-2 and AAC( 6’)-1b probes.

关 键 词：肺心病 绿脓杆菌 分型 质粒 DNA 分子杂交 

分 类 号：R541.503[医药卫生—心血管疾病]