气相色谱鉴定分支杆菌对比实验  

Contrastive experiment of gas chromatograpby deter-mining Mycobacteria

作　　者：翟秉详[1] 张丽杰[1] 闫秀印 戴跃祥 杨立学[1] 

机构地区：[1]河北省胸科医院,石家庄市卫生防疫站

出　　处：《中华结核和呼吸杂志》1995年第6期344-347,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：采用毛细管柱气相色谱（ＧＣ）检测分支杆菌细胞脂肪酸（ＦＡ）的方法，通过制备２６种参考菌株ＧＣ图谱（原冻干菌株及其移种改良罗氏培养基生长３～４周菌株）对临床分离的５４株非结核分支杆菌以ＧＣ法与传统生物学鉴定法进行对比，两者符合率达９４％；观察了结核分支杆菌和牛分支杆菌临床分离株ＧＣ图谱变化与耐药性的关系。认为ＧＣ法鉴定临床分离分支杆菌目前采取同时观察细菌生长速度、产色情况以及在对硝基苯甲酸（ＰＮＢ）、２－羧酸肼噻吩（ＴＣＨ）培养基上生长情况为妥。sing capillary column gas chromatography(GC)technique for detecting cellular fatty acid,the test of theGC chromatograms of 26 reference Mycobacteria strains(freeze-dried strains and transfer growed strains at theimproved Lowenstein-Jensen culture for three or fourweeks)was performed.On the basis of the test,a com-panison was made between the GC method and the con-ventional method for determination of 54 strains of non-tuberculous mycobacteria which were separated fromclinical specimens, and the conformational rate of thetwo methods was 94 percent.At the same time,the re-lationship between the drug resistance and the changesof GC chromatograms of M,tuberculosis and M. boviswhich were separated from clinical samples was ob-served.The results demonstrated that the GC methodwould achieve beffer results if combined with be bacte- rial growing speed,the condition of producing colourand the condition of growing at PNB and TCH cultureduring determining the mycobacterium strains whichwere separated from clinical specimens.

关 键 词：结核杆菌 分枝杆菌属 鉴定 气相色谱法 脂肪酸 

分 类 号：R378.911[医药卫生—病原生物学]