枯草芽孢杆菌纤溶酶基因在转基因烟草组织中的分泌表达  

Expression and Secretion of Bacillus subtilis Fibrinolytic Enzymic (BSFE) Gene from Transgenic Tobacco Tissues

作　　者：王瑞刚[1,2] 徐萍[3] 李高[4] 张子义[1] 王彦[1] 吴自荣[3] 王水平[3] 

机构地区：[1]内蒙古农业大学生物工程学院 [2]华东师范大学生命科学学院,上海200062 [3]华东师范大学生命科学学院 [4]北京市园林局颐和园管理处

出　　处：《西北植物学报》2005年第7期1383-1388,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市科委重大项目"安舒克栓酶的药效及工艺研究"(985419000-3)

摘　　要：克隆枯草芽孢杆菌纤溶酶(Bacillussubtilisfibrinolyticenzyme,BSFE)基因及其前导肽序列。通过农杆菌EHA105介导转化,获得转基因烟草植株。其BSFE的表达水平为叶片42.97±28.59U·g-1FW、茎15.14±10.57U·g-1FW和根25.55±14.71U·g-1FW。其内源BSFE信号肽可在转基因烟草中行使蛋白转运功能,使BSFE具有分泌表达特性。这一系统可用于建立利用植物组织分泌表达外源蛋白的系统模型。Bacillus subtilis fibrinolytic enzyme (BSFE) gene with its signal peptide and propeptide sequece were cloned.Transgenic tobacco was obtained by Agrobacterium strain EHA105 mediated transformation.The transgenic tobacco could secret target protein from leaf,stem or root.The amount of BSFE expressed from transgenic tobacco reached 42.97±28.59 U·g^(-1) FW in leaf,15.14±10.57 U·g^(-1) FW in stem and 25.55±14.71 U·g^(-1) FW in root respectively.This system could be used as a model for expression and secretion of exogenous proteins by plant tissues.

关 键 词：枯草芽孢杆菌纤溶酶 转基因 烟草 分泌表达 

分 类 号：Q786[生物学—分子生物学]