急性期升高血压对大鼠局灶性脑缺血损伤的影响  

The influence of induced hypertension in acute phase on focal cerebral ischemia injury

作　　者：贾建平[1] 矫黎东[1] 周冀英[1] 赵洪波[2] 

机构地区：[1]首都医科大学宣武医院神经内科,北京100053 [2]北京大学医学部基础学院药理系

出　　处：《中华医学杂志》2005年第26期1813-1815,共3页National Medical Journal of China

基　　金：国家自然科学基金资助项目(30370493)

摘　　要：目的探讨急性期升高血压治疗对局灶性脑缺血大鼠脑梗塞体积和脑血流的影响。方法健康成年雄性SD大鼠36只,被随机分为单纯缺血3、4、6h组及缺血2、3、5h升高血压1h组,共6组,每组6只。制备大脑中动脉梗死模型,采用激光多普勒血流仪测量大鼠局灶性脑缺血各时相皮层缺血中心边缘区局部脑血流(rCBF),并于各时间点取大鼠脑切片,红四氮唑染色后,计算机图像分析系统测量脑梗死体积。结果缺血2、3、5h升压组大鼠rCBF分别恢复至基础rCBF的(81.8±3.1)%、(56.0±2.1)%和(38.8±2.0)%。缺血2h、3h升压组脑梗死体积分别为(14±7)mm3、(90±24)mm3,均显著小于相应的单纯缺血组比较梗塞体积显著缩小(均P<0.05)。结论改善缺血中心边缘区脑血流可能是急性期升压疗法治疗局灶性脑缺血损伤的主要机制之一。Objective To study the influence of induced hypertension in acute phase on the infarct volume and regional cerebral blood flow(rCBF) during focal cerebral ischemia in rats. Methods The adult rat′s models of permanent focal cerebral ischemia were established by permanent middle artery occlusion. Thirty-six rats were divided six groups. The rCBF of cortical penumbra during focal cerebral ischemia was determined by Laser-Doppler Flowmetry. After experiment was over,the brain was TTC stained and the infarct bolume was calculated by computer image analytic system. Results The rCBF was resumed by (81.8±3.1)%、(56.0±2.1)%和(38.8±2.0)% in induced hypertension groups compared with the basic rCBF. The Volume of infarction was (14±7)mm^3、(90±24)mm^3 seperately in group induced hypertension after 2 and 3 hour ischemia. Induced hypertension during 3 hours could reduce infarct volume significantly. Conclusion To improve rCBF in penumbra may be one of protective mechanism of induced hypertension on brain ischemia.

关 键 词：局灶性脑缺血损伤 升高血压 急性期 计算机图像分析系统 大鼠局灶性脑缺血 激光多普勒血流仪 大脑中动脉梗死 红四氮唑染色 rCBF 脑梗塞体积 高血压治疗 局部脑血流 脑梗死体积 缺血大鼠 SD大鼠 鼠脑切片 升压疗法 边缘区 

分 类 号：R544.1[医药卫生—心血管疾病]