血糖及皮肤组织糖含量对大鼠浅二度烫伤创面愈合影响的实验研究  

Effects of the cutaneous and blood contents of glucose on wound healing in diabetic rats with superficial partial thickness scalding

作　　者：陆树良[1] 乔亮[1] 谢挺[1] 杨奕敏[1] 金曙雯[1] 青春[1] 

机构地区：[1]上海第二医科大学附属瑞金医院上海市烧伤研究所,200025

出　　处：《中华医学杂志》2005年第27期1899-1902,共4页National Medical Journal of China

基　　金：国家重点基础研究发展规划资助项目(G1999054205)

摘　　要：目的研究血糖及皮肤组织糖含量对浅二度烫伤创面愈合的影响。方法96只清洁级SD大鼠随机分成正常组和以STZ诱导的速发型糖尿病组,并造成10%体表总面积(TBSA)的浅二度烫伤模型,分别测定伤前、伤后第1天、3天、5天、7天、10天和14天的血糖、皮肤或创面组织糖含量;大体和组织学动态观察创面愈合过程;伤前和伤后3d、7d和14d检测表皮细胞增殖周期。结果糖尿病大鼠血糖浓度明显高于正常组(27.28mmol/L±0.80mmol/Lvs4.65mmol/L±0.14mmol/L,P<0.01);皮肤组织创面中局部的糖含量与血糖变化有显著的相关性(r=0.881,P<0.05),糖尿病组大鼠浅二度烫伤创面局部糖含量明显高于正常烫伤组(14.2mmol/L±3.0mmol/gvs4.5mmol/L±0.5mmol/g,P<0.01)。糖尿病烫伤创面愈合明显延迟,新生上皮明显减少,表皮细胞进入S期和G2/M期百分比增殖较正常大鼠显著减少。结论糖尿病大鼠局部创面组织糖含量增加与血糖变化密切相关,而创面局部糖含量的增加与创面愈合延迟存在着不可分割的联系。高糖环境可抑制浅二度烫伤创面表皮细胞增殖。Objective To study the effect of the cutaneous and blood contents of glucose on wound healing in diabetic rats with superficial partial thickness scalding. Methods 96 Sprague-Dawley rats were randomized into control group and STZ-induced diabetic group, inflicted with superficial partial thickness scalding of 10%TBSA on the back. The glucose content in the blood and wound tissue were evaluated before injury and at day 1, day 3, day 5, day 7,day10 and day14 after injury. Wound healing process in the rats was dynamically observed by naked eyes and histologically examined. The cell cycles of keratinocytes from wound site were determined before injury and at day 3, day 7 and day10 after injury. Results The concentrations of blood glucose in diabetic wound was significant increased than normal group(27.28 mmol/L±0.80 mmol/L vs. 4.65 mmol/L±0.14 mmol/L,P<0.01). The content of local glucose in wound tissue were significantly correlated with that in the blood(r=0.881, P<0.05). When compared with the control group, wound healing of the diabetic rats were delayed with the characteristics of impaired epithelialization and decreased.percentages of S stage and G_2/M stages of keratinocytes. Conclusion Local glucose content in diabetic wound was varied with blood glucose concentration. The increased glucose concentration in diabetic wound was attributed to the impaired wound healing in diabetes. High glucose concentration could inhibit the epithelialization and decrease the keratinocyte proliferation.

关 键 词：烫伤创面愈合 糖含量 实验研究 mol/L 清洁级SD大鼠 糖尿病大鼠 细胞增殖周期 皮肤组织创面 创面愈合延迟 表皮细胞增殖 创面组织 血糖变化 STZ诱导 型糖尿病 烫伤模型 愈合过程 动态观察 血糖浓度 正常大鼠 高糖环境 

分 类 号：R644[医药卫生—外科学]