哮喘大鼠信息传递与转录活化因子6的表达和地塞米松对其表达的影响  

Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone

作　　者：李昌崇[1] 叶乐平[1] 陈小芳[1] 李绍波[1] 蔡晓红[1] 董琳[1] 罗运春[1] 张正霞[1] 

机构地区：[1]温州医学院附属育英儿童医院呼吸科,325027

出　　处：《中华儿科杂志》2005年第7期521-525,共5页Chinese Journal of Pediatrics

基　　金：浙江省自然科学基金(301485)

摘　　要：目的研究哮喘大鼠支气管信息传递与转录活化因子6(STAT6)的表达和地塞米松(DXM)对其表达的影响。方法30只体重120～180g的二级幼年雄性SD大鼠,随机分为3组:正常对照组(A组)、哮喘组(B组)、地塞米松组(DXM组)。对支气管肺泡灌洗液(BALF)进行细胞总数、嗜酸性粒细胞(EOS)计数和分类计数;应用双抗体夹心酶联免疫吸附试验法测定BALF和血清中白细胞介素4(IL4)浓度;采用免疫组化法和原位杂交法分别检测支气管STAT6蛋白和STAT6mRNA的表达。结果(1)B组支气管STAT6蛋白和STAT6mRNA表达[(0.171±0.025),(0.180±0.013)]均高于A组[(0.082±0.022),(0.091±0.012)]和DXM组[(0.114±0.013),(0.114±0.010)](均为P<0.01),其主要表达细胞是上皮细胞;(2)支气管STAT6蛋白、STAT6mRNA分别与BALF中的IL4浓度呈显著正相关(r=0.664、0.785,P<0.01),STAT6蛋白、STAT6mRNA分别与BALF中的EOS绝对值呈显著正相关(r=0.869、0.884,P<0.01)。结论哮喘大鼠支气管有STAT6的较强表达,上皮细胞是其主要表达细胞;地塞米松可以下调STAT6的表达,可能为其抑制哮喘气道炎症形成的重要作用机制。Objective To study the expression of signal transducer and activator of transcription 6 and its mRNA in rat asthma model and the modulatory effect of dexamethasone (DXM). Methods Thirty male SD rats were randomly divided into three groups: the control group, asthma group and DXM group. The rats in each group were sacrificed 24 h after the last challenge. In the experiment, the rat model of asthma was established by ovalbumin (OVA) challenge method. The lung tissue was taken from the left lung, and bronchoalveolar lavage fluid (BALF) was collected from the right lung. The total cell numbers, eosinophils (EOS) count and differentiated cell counts in BALF were performed on different count fluids. The concentrations of IL-4 in serum and BALF were measured by using sandwich ELISA. The protein expressions of STAT6 were detected with immunohistochemical techniques. The mRNA expressions of STAT6 were detected with in situ hybridization. Results (1)The total cell counts in BALF, the absolute counts of EOS, and the ratios of eosinophils to the total cell numbers (EOS%) of asthma group were all significantly higher than those of the control group (P<0.01). The total cell counts in BALF, the absolute counts of EOS, and EOS% of DXM group were all significantly lower than those of asthma group (P<0.01). (2)The concentrations of IL-4 in BALF and serum of asthma group [(25.7±7.4) ng/L, (34.2±10.5) ng/L] were significantly higher than those of control group [(8.6±3.0) ng/L, (12.1±2.9) ng/L](P<0.01). The concentrations of IL-4 in BALF and serum of DXM group were significantly lower than those of asthma group.(3)Immunohistochemistry showed that the protein content of STAT6 around the bronchus of asthma group (0.171±0.025) was significantly higher than that of the control group (0.082±0.022) ((P<)0.01), while that of DXM group (0.114±0.013) was significantly lower than that of asthma group. The epithelial cells were the cells. In situ hybridization showed that the mRNA expression of STAT6 around the bronchus of asthma group (

关 键 词：转录活化因子 地塞米松 信息传递 酶联免疫吸附试验法 STAT6 支气管肺泡灌洗液 嗜酸性粒细胞 BALF mRNA表达 哮喘气道炎症 上皮细胞 表达细胞 正常对照组 白细胞介素 双抗体夹心 原位杂交法 免疫组化法 SD大鼠 细胞总数 

分 类 号：R725.6[医药卫生—儿科]