多聚酶链反应技术在斑点热群立克次体流行病学调查中的应用  

The Application of PCR to Epidemiological Study on Spotted Fever Group Rickettsiae

作　　者：张健之[1] 范明远[1] 毕德增[1] 孙福祥 程昭祥 兰福春 崔文富 孙秀英 只相国 林碧瑚 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所,黑龙江省虎林县卫生防疫站,饶河县卫生防疫站,绥芬河卫生检疫局,绥芬河市卫生防疫站,黑龙江省卫生防疫站,海南

出　　处：《中华流行病学杂志》1995年第1期25-28,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金;卫生部科研基金资助课题

摘　　要：作者首次用来自立氏立克次体（Ｒ．ｒｉｃｋｅｔｔｓｉｉ，Ｒｒ）１９０ＫＤａ蛋白抗原基因序列设计的一对引物扩增从黑龙江、河北、海南、北京采集的蜱、蜱卵、幼蜱、蜱粪及啮齿动物脏器中斑点热群立克次体ＤＮＡ。结果从上述标本中均检测出了斑点热群立克次特异的５３２ｂｐ大小的ＤＮＡ片段，该结果部分与同期进行的病原分离结果一致。故认为，ＰＣＲ技术是一种快速、敏感、特异的斑点热群立克次体流行病学调查方法。It was the first time that a primer pairs derived from the 190KDa protein antigen gene of R. rickettsii were used to amplify SFGR DNA in ticks.ticks. tick ova,larva ,tick faeces and rodent organs which were collected in Hebei,Heilongjiang,Hainan nd Beijing. A 532bp fragment was respectively amplified from above samples.The results were partially in concordance with data obtained through rickettsiae isolation. It was suggestedthat PCR is a rapid,specific,sensitive and practical method for detection of SFGR in endemic foci.

关 键 词：多聚酶链 SFGR 立克次体 蜱 流行病学 

分 类 号：R376[医药卫生—病原生物学]