应用羧化胶乳凝集试验诊断人、畜布鲁氏菌病的研究  

Study on Carboxylated Latex Agglutiuation Test for Serodiagnosis of Human aud Animai Brucellosis

作　　者：鲁齐发[1] 王晓英[1] 郝宗宇 宋桂梅 张伟[1] 郭宝岚[1] 李兰玉[1] 冯立忠 严泽民 张殿柱 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所,河南省地方病防治研究所,黑龙江省卫生防疫站,山西省卫生防疫站,青海省地方病防治研究所,青海省兽医总站

出　　处：《中华流行病学杂志》1995年第2期92-94,共3页Chinese Journal of Epidemiology

基　　金：国家自然科学基金

摘　　要：应用以化学交联法制备的布鲁氏菌１６Ｍ抗原－羧化胶乳制剂的羧化胶乳凝集试验（ＬＡＴ）、试管凝集试验（ＳＡＴ）、虎红平板凝集试验（ＲＢＰＴ）以及酶联免疫吸附试验（ＥＬＩＳＡ）、半胱氨酸凝集试验（ＣＹＴ），对在四省布鲁氏菌病疫区收集的部分人、畜血清中布氏菌抗体进行对比检测。结果表明，ＬＡＴ较之ＲＢＰＴ具有更高的特异性；与ＳＡＴ相比，无论阳性或阴性符合率均较一致。另外，ＬＡＴ可检测血清中ＩｇＧ、ＩｇＭ两类布氏菌特异性抗体。因而本试验对人、畜布病的诊断具有特异、敏感和简便的优点，更宜在基层推广应用。carboxylated latex agglutination test was developed for detecting antibodies in sera from 372 humanand animal brucelIosis.Of which 94 sera from human was detected by ELISA. The latex was chemical-linkedwith B. melitensis 16M and extracted with 5% NaCl.Comparing with RBPT in detecting antibodies with bru-cellosis,the LAT was more stable and specific;the positive or negative results detected by LAT were consis-tent with those by SAT and ELISA. EAT may detect both IgG and IgM antibodies to Brucella in Human and animal.It is concluded that LATis stable,specific,sensitive and practicible for the serodiagnosis of brucellosis.

关 键 词：布鲁氏菌病 羧化胶乳凝集 人 家畜 诊断 

分 类 号：R516.704[医药卫生—内科学] S855.12[医药卫生—临床医学]