聚合酶链反应对肠结核和克隆病的诊断价值  

THE VALUE OF POLYMERASE CHAIN REACTION ASSAY IN THE DIAGNOSIS OF INTESTINAL TUBERCULOSIS AND DIFFERENTIATION FROM CROHN'S DISEASE

作　　者：甘华田[1] 欧阳钦[1] 步宏[1] 陈德珍[1] 李甘地[1] 李蜀华[1] 杨秀英[1] 

机构地区：[1]华西医科大学附属第一医院内科,华西医科大学附属第一医院病理科

出　　处：《中华内科杂志》1995年第1期30-33,共4页Chinese Journal of Internal Medicine

摘　　要：肠结核的诊断及其与克隆病的鉴别诊断颇为困难。在本研究中我们探讨了聚合酶链反应（ＰＣＲ）技术在这两方面的价值。３６例肠结核、２６例克隆病标本被纳入本研究。引物来自结核杆菌特异重复插入序列ＩＳ６１１０。其产物特导性通过Ｓａ１Ｉ限制性内切酶消化和地高辛标记的探针经Ｓｏｕｔｈｅｒｎ杂交证实。结果显示：在３６例肠结核中，２７例ＰＣＲ阳性１２６例克隆病中无１例阳性。从而证实了ＰＣＲ对肠结核是一种快速、敏感和特异的病原学诊断方法；并对肠结核与克隆病的鉴别诊断具有极高价值。t is difficuIt to make precise dlagnosis for intestinal tuberculrels and dlfferentiate it from Crohn's disease,For evaluating the efficacy of PCR assay in these two aspects, 36 specimens of intestinal tuberculo-sis and 26 of Croh's disease frotn surgical rearections and endoscopic blopsies were subjected to PCR assay. Ohigonuceotides derived from IS 6110 guwnce,whlch is a repeatdin M, tuberculosis chromosome and highly specific for the M;tuharculosis complex,were used as a ptimer,The sMificity of PCR prloducts was confirmed by digestion with Sal I restrictive endonuclease and southern blot hybrldiz1ltion by using digoxigenin- labeled probe,The results showed that M,tuberculcois DNA was identlfied in 27 uf the 36specimens of intestinal tuberculosis,but none in those of 26 Crohn's disease,In conclusion, PCR is a rapld,eensItive and stwific methed for the diagnosis of intestinal tuberruIosis,and it is also valuable in dif-ferentiating intestinaI tuberculosis from Ctohn's disease.

关 键 词：肠结核 克隆病 聚合酶链反应 诊断 鉴别诊断 

分 类 号：R524.04[医药卫生—内科学] R574.620.4[医药卫生—临床医学]